Abstract Number: VPB1116
Meeting: ISTH 2022 Congress
Background: Heparin-induced thrombocytopenia (HIT) is a drug reverse disorder (happening in up to 5% of patients) that could lead to lethal consequences. The patient is confirmed with HIT if HIT antibodies (Abs) are present. Recently, HIT Abs were detected also in clinically ill Covid-19 and VITT patients. Early detection of HIT is crucial, but the widely used immunoassays provide only ~50% accuracy while functional assays required fresh platelets, which can only be performed in limited laboratories. Thus, the development of a simple method but accurate detection for confirmation of HIT Abs is highly desired.
Aims: We aim to discover cell membranes as novel materials to improve the detection of HIT Abs.
Methods: We used the laser scanning confocal microscope (LSCM) to visualize and confirm the binding of HIT Abs (including monoclonal Abs and patient’s sera) on breast cancer cell line MDA-MB-231. Flow cytometry was used to quantify the binding of antibodies via bound PF4 or PF4/Heparin complexes on the cells. Optical density caused by bindings of HIT Abs to cells was detected by integrating the cells into the ELISA setup.
Results: We found that the MDA-MB-231 cell line allows bindings of HIT Abs via platelet factor 4 (PF4) or PF4/Heparin complexes. Bound PF4 on cells allowed better binding of HIT Abs than the bound PF4/H complexes. Heparin inhibits HIT antibody bindings to cells. Importantly, non-HIT Abs also bind to the cells through PF4 but are much weaker than the binding of the HIT Abs. The different binding patterns between HIT and non-HIT Abs could be clearly distinguished.
Conclusion(s): The MDA-MB-231 cell line has a great potential to distinguish HIT from non-HIT Abs through PF4 but not PF4/H complexes.
To cite this abstract in AMA style:Chen L, Nguyen H. A novel material for the improvement of HIT detection [abstract]. https://abstracts.isth.org/abstract/a-novel-material-for-the-improvement-of-hit-detection/. Accessed September 27, 2022.
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