Abstract Number: PB0342
Meeting: ISTH 2021 Congress
Background: Fibrinogen is the most abundant coagulation factor on plasma, a key component of hemostasis and inflammation, and an acute-phase protein. In acquired conditions leading to hypofibrinogenemia – e.g., snakebite envenomation, trauma-induced coagulopathy, disseminated intravascular coagulation, sepsis and liver dysfunction –, the rapid determination of fibrinogen levels is essential to provide correct diagnosis and treatment to patients. Current assays for fibrinogen measurement have limitations, such as expensive costs, time-consuming manipulations and the requirement of centrifugation steps to obtain plasma samples. Therefore, to develop and validate new point-of-care diagnostic tests for fibrinogen assay is essential.
Aims: To develop a novel and rapid paper-based test to assay fibrinogen levels in whole blood, taking into consideration that during coagulation activation fibrinogen is clotted into a fibrin network that is semi-permeable to dye solutions.
Methods: Procedures involving animals were approved by IACUC. The flow assay cassette was constructed using a 3D printer, lateral flow plasma separation pads were used to separate red blood cells from plasma, and filter paper strips were the support for observing the migration distance of a methyl green dye solution. Mice were injected with saline (controls) or snake venom to evaluate hypofibrinogenemia. After 3 h, whole blood (30 µL) was collected from the lateral saphenous vein, and added to the diagnostic device. After 3 minutes, the dye solution was added, and the travel distance was then recorded at 2, 5 and 10 minutes.
Results: Envenomed mice had hypofibrinogenemia compared to control mice, and fibrinogen levels were inversely proportional to the travel distance of the dye (r=-0.9, p<0.050).
Conclusions: The test was effective in detecting differences in fibrinogen levels in whole blood samples. We are currently evaluating the performance of this diagnostic test with other samples from F9, F8 and Vwf knockout mice, as well as in human blood samples.
Funding: FAPESP, CNPq, CAPES, Fundação Butantan.
To cite this abstract in AMA style:Sachetto AT, Picon NT, Morgado CB, Santoro ML. A Novel Paper-based Test for Diagnostic of Fibrinogen Levels on Whole Blood [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/a-novel-paper-based-test-for-diagnostic-of-fibrinogen-levels-on-whole-blood/. Accessed December 7, 2021.
« Back to ISTH 2021 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/a-novel-paper-based-test-for-diagnostic-of-fibrinogen-levels-on-whole-blood/