Abstract Number: PB0793
Meeting: ISTH 2020 Congress
Background: Snakebite envenomation – a tropical neglected disease with over 2 million accidents per year, which show a high rate of morbidity – is a type of acquired thrombo-hemorrhagic disease. In Brazil, bites from Bothrops snakes have a complex pathophysiology, inducing thrombocytopenia, consumption coagulopathy with hypofibrinogenemia, secondary hyperfibrinolysis, and bleedings in victims. The treatment with antivenom has limitations and the management of the local effects of the snakebite, as local hemorrhage and the inflammatory reaction, still presents a great challenge. On that account, we searched for potential new ancillary therapeutic compounds, as rutin, a safe natural flavonoid, easily available, inexpensive and with broad range of beneficial activities.
Aims: To verify the potential of rutin and its water-soluble form, rutin succinate, as a treatment for the hemostatic disorders and local hemorrhage induced by Bothrops jararaca snake venom (BjV).
Methods: All procedures involving animals were in accordance with IACUC. C57BL/6 mice were injected with rutin or rutin succinate (negative controls, i.v.), BjV (1.6 mg/kg b.w., s.c., positive control), or BjV followed by i.v. injection of rutin or rutin succinate (14.4 mg/kg b.w. i.v.) 10 min later. At 6, 24 or 48 h after envenomation, blood was collected and local hemorrhage was evaluated.
Results: Mice showed characteristic hemostatic disturbances of snake envenomation as thrombocytopenia, hypofibrinogenemia, and hemorrhage at the site of BjV injection. Treatment with rutin or rutin succinate induced a faster recovery of fibrinogen levels at 24 h. Moreover, when administered as a treatment, rutin succinate decreased the local hemorrhage at 48 h.
Conclusions: Our results indicate that rutin and rutin succinate ameliorated local and systemic disturbances induced by the envenomation, showing thereby a great potential as ancillary treatments for snake envenomation and possibly to other thrombo-hemorrhagic diseases. Funding: FAPESP, CNPq, CAPES, Fundação Butantan.
To cite this abstract in AMA style:Sachetto ATA, de Souza AO, Santoro ML. A Novel Therapeutic Approach for Acute Thrombo-hemorrhagic Disorders Evoked by Snake Venom: Potential of Rutin and Rutin Succinate [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/a-novel-therapeutic-approach-for-acute-thrombo-hemorrhagic-disorders-evoked-by-snake-venom-potential-of-rutin-and-rutin-succinate/. Accessed January 28, 2022.
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