Abstract Number: PB0052
Meeting: ISTH 2020 Congress
Background: Vascular diseases are the major cause of mortality and morbidity and associated with high societal costs. The understanding of the pathological mechanism’s underlying cardiovascular remodeling; although critical but essential for the development of innovative therapies for vascular diseases. Left-ventricular cardiac hypertrophy is a well-established precursor of heart failure with the ominous prognosis.
Aims: To develop the surgical procedure that can easily and efficiently replicate this condition is too critical, and is significantly important for the better understanding of the pathophysiological mechanisms associated with this vascular disease.
Methods: Constriction of the aorta in mice is a highly accepted method for the creation of pressure overload-induced cardiac hypertrophy model. The two microsurgical procedures available for the successful development of the disease model are aortic constriction at the level of transverse aorta and o-ring aortic banding using the ascending aorta.
Results: The transverse aortic constriction has many advantages over the other available technique which is significantly highlighted in this paper. Using transverse aorta, though the degree of constriction is widely dependent on the diameter of the gauge needle. Our main purpose is to establish a comparison between these two surgical procedures to highlight the highly reproducible and precise method to study the mechanism of the disease progression as well as cardiac remodeling.
Conclusions: To summarize, both techniques have some advantages and disadvantages to one and other. Many studies reports that TAC is more efficient and it generates consistent and homogenous stenosis of aortic arch. Therefore, this technique provides stable and fully controlled aortic constriction. So, this microsurgical procedure can be easily completed with basic surgical understanding.
To cite this abstract in AMA style:Rashid A. A Study on Pressure Overload-Induced Cardiac Hypertrophy Model in Mice by Applying Transverse Aortic Constriction and O-Ring Ascending Aortic Banding Technique: A Comparative Analysis [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/a-study-on-pressure-overload-induced-cardiac-hypertrophy-model-in-mice-by-applying-transverse-aortic-constriction-and-o-ring-ascending-aortic-banding-technique-a-comparative-analysis/. Accessed January 26, 2022.
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