ISTH Congress Abstracts

Official abstracts site for the ISTH Congress

MENU 
  • Home
  • Congress Archive
    • ISTH 2022 Congress
    • ISTH 2021 Congress
    • ISTH 2020 Congress
  • Resources
  • Search

Activation of ERK Signaling Pathway by Plasma IgG in Venous Thromboembolism Patients without Antiphospholipid Antibodies

S. Bouvier1,2,3, S. Bastide4, M. Fortier1, J. Laurent5, E. Mercier1,2,3, E. Nouvellon1, A. Remy1, G. Lavigne1, I. Quéré6, A. Pérez-Martin2,5, J.-C. Gris1,2,3

1Nîmes University Hospital, Department of Haematology, Nimes, France, 2Montpellier University, Research Laboratory EA 2992, Montpellier, France, 3Faculty of Pharmaceutical and Biological Sciences, Montpellier University, Department of Haematology, Montpellier, France, 4Nîmes University Hospital, Department of Biostatistics, Public Health and Innovation in Methodology, Nimes, France, 5Nîmes University Hospital, Department of Vascular Medicine, Nimes, France, 6Montpellier University Hospital, Department of Vascular Medicine, Montpellier, France

Abstract Number: PB2013

Meeting: ISTH 2020 Congress

Theme: Vascular Biology » Innate and Adaptive Immunity

Background: Venous thromboembolism (VTE) is still a huge challenge for Public Health. Autoimmune diseases play an increasing role in human pathologies, antiphospholipid antibody (aPL) syndrome being one of the models for VTE pathophysiology. aPL have been found to activate several signalling pathways, but we lack data on circulating antibodies-related cell activities in aPL-negative patients with VTE.

Aims: We hypothesized that certain circulating immunoglobulins G (IgG) in VTE patients might stimulate the signalling pathways involved in monocyte activation, even in aPL-negative patients (clinicaltrials.gov identifier: NCT02713581).

Methods: Plasma IgG from 13 VTE patients and 20 controls were purified by column chromatography. These purified IgG were added to a THP-1 monocyte culture medium, and proteins were extracted for subsequent western-blot analysis. Quantification of actin-normalized phospho-ERK, phospho-P38 and phospho-AKT allowed us to characterize the monocyte activation profile for each patient. Modulation of signalling pathways between groups was assessed per pathway by two-way ANOVA which also takes into account the experiment effect.

Results: Our results show a significant increase in the phospho-ERK/Actin ratio (p< 0.0001). There was no group effect concerning total protein ERK, demonstrating that activation was only due to an increase in ERK-phosphorylation (p=0.419). No significant differences were found for the other pathways (p=0.964 and p=0.174 respectively for phopsho-p38 and phospho-AKT).

Conclusions: Our pilot study shows that total IgG from patients with a history of VTE may activate the ERK pathway in the absence of antiphospholipid antibodies. The impact of this IgG-related activity on individual risks of VTE deserves to be investigated.

To cite this abstract in AMA style:

Bouvier S, Bastide S, Fortier M, Laurent J, Mercier E, Nouvellon E, Remy A, Lavigne G, Quéré I, Pérez-Martin A, Gris J-. Activation of ERK Signaling Pathway by Plasma IgG in Venous Thromboembolism Patients without Antiphospholipid Antibodies [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/activation-of-erk-signaling-pathway-by-plasma-igg-in-venous-thromboembolism-patients-without-antiphospholipid-antibodies/. Accessed September 29, 2023.

« Back to ISTH 2020 Congress

ISTH Congress Abstracts - https://abstracts.isth.org/abstract/activation-of-erk-signaling-pathway-by-plasma-igg-in-venous-thromboembolism-patients-without-antiphospholipid-antibodies/

Simple Search

Supported By:

Takeda logo

ISTH 2022 Congress site

Visit the official web site for the ISTH 2022 Virtual Congress »

  • Help & Support
  • About Us
  • Cookies & Privacy
  • Wiley Job Network
  • Terms & Conditions
  • Advertisers & Agents
Copyright © 2023 John Wiley & Sons, Inc. All Rights Reserved.
Wiley