Abstract Number: VPB0611
Meeting: ISTH 2022 Congress
Background: SARS-CoV-2 tropism for the ACE2 receptor, along with the multifaceted inflammatory reaction, is likely to drive the generalized hypercoagulable state seen in patients with COVID-19.
Aims: According to our previously published bioinformatics analysis, we aimed to analyze the diagnostic and predictive utility of miRNAs regulating ACE2 network (miR-26b-5p, miR-10b-5p, miR-302c-5p, hsa-miR-200b-3p, hsa-miR-124-3p) in patients with COVID-19
Methods: We determined the expressions of ACE2-related-miRNAs in 79 hospitalized COVID-19 patients and 32 healthy volunteers by PCR and monitored miRNAs patterns during the acute phase of COVID-19, as well as the prognostic potential of these miRNAs as biomarkers.
Results: The expression levels of miR-26b-5p in COVID-19 patients were found lower at the baseline, 7 and 21-days after admission. compared to the healthy controls (p < 0.0001 for all time points). Similarly, miR-10b-5p expression levels were lower at the baseline and 21-days post admission in COVID-19 patients when compared to the healthy individuals (p=0.001 in both time points). Moreover, expression levels of this miRNA were higher 7-days post-admission when compared to the baseline (p=0.003). According to the ROC curve analysis, low miR-200b-3p expression presents predictive utility in assessment of the hospital length of stay and/or death (AUC:0.730, p=0.002). According to the multivariable logistic regression model, low delta miR-200p expression, together with diabetes mellitus (DM), are independent predictors of increased hospital length of stay and/or death (OR: 5.775; 95% CI, 1.572-21.214; p=0.008 and OR: 4.888; 95% CI, 1.001-23.858; p=0.050, respectively).
Conclusion(s): This study enabled us to better characterize changes in gene expression and signaling pathways related to COVID-19 thrombosis. In this study we identified, characterized and validated miRNAs which could serve as novel, thrombosis-related biomarkers of the COVID-19, can be used for early stratification of patients and prediction of severity of infection development in an individual.
To cite this abstract in AMA style:Wicik Z, Eyileten C, Jarosz-Popek J, Jakubik D, Nowak A, Wolska M, Shahzadi A, Martins-Jr D, Simões S, Siller-Matula J, Postula M. Alteration of circulating ACE2 regulating microRNAs profiles in patients with COVID-19 [abstract]. https://abstracts.isth.org/abstract/alteration-of-circulating-ace2-regulating-micrornas-profiles-in-patients-with-covid-19/. Accessed September 29, 2023.
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