Abstract Number: PB1635
Meeting: ISTH 2020 Congress
Background: Platelets play an important role in coagulation and are known to be crucial in the development of arterial thrombosis. An algorithm based on thrombin generation (TG) was created to study thrombin dynamics more in detail. This computational approach takes into account both pro- and anticoagulant mechanisms during TG, as well as plasma levels of fibrinogen, α2Macroglobulin (α2M) and antithrombin. By quantifying prothrombin conversion and thrombin inactivation, more information is gained about the pro- and anticoagulant processes that underlie TG in platelet rich plasma (PRP). Especially for patients with a platelet disorder or for patients taking drugs that affect platelet function, it is interesting to investigate the overall thrombin potential kinetics in PRP.
Aims: To determine the effect of platelet count on TG and thrombin dynamics in PRP.
Methods: PRP was collected from 117 healthy individuals. PRP TG was measured undiluted and diluted to a platelet concentration of 50*109/L and 150*109/L. Pearson correlation test was used to determine correlations between parameters.
Results: Thrombin dynamics was established in PRP of 117 healthy individuals. Peak, velocity index and the maximum rate of prothrombin conversion increased linearly with platelet count, but endogenous thrombin potential reached a maximum at 150*109/L. Correlation analysis indicated that the platelet count mainly affected the rate of prothrombin conversion. Inhibition of thrombin by antithrombin and α2M increased with increasing TG, but the ratio of inhibition by antithrombin or α2M remained the same independently of the total thrombin formed.
Conclusions: TG and thrombin dynamics were assessed in PRP of healthy donors and demonstrated that increasing the platelet count significantly affected the rate of prothrombin conversion and TG, rather than the total amount of thrombin formed. As venous thromboembolism (VT) can be caused by insufficient attenuation of coagulation by the anticoagulants, patients with high platelet counts could have an increased risk of developing VT.
To cite this abstract in AMA style:Yan Q, Ninivaggi M, de Laat B, de Laat-Kremers R. An Elevated Platelet Count Increases the Rate of Prothrombin Activation, but Not the Total Amount of Thrombin Formed [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/an-elevated-platelet-count-increases-the-rate-of-prothrombin-activation-but-not-the-total-amount-of-thrombin-formed/. Accessed October 1, 2023.
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