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Analysis of Coagulation Factors in Fresh-frozen Plasma

1Department of Laboratory Medicine, North Estonia Medical Centre, Tallinn, Estonia, 2Department of Health Technologies, Tallinn University of Technology, Tallinn, Estonia, 3Research Program Unit of Systems Oncology, Helsinki University Hospital, Unit of Coagulation Disorders, Helsinki, Finland, 4Blood Centre, Foundation Pärnu Hospital, Pärnu, Estonia, 5Blood Service, Ida-Viru Central Hospital, Kohtla-Järve, Estonia, 6Faculty of Medicine, Institute of Biomedical Sciences, Department of  Physiology, Biochemistry, Microbiology and Laboratory Medicine, Vilnius University, Vilnius, Lithuania

Abstract Number: PB0072

Meeting: ISTH 2021 Congress

Theme: Coagulation and Natural Anticoagulants » Coagulation Factors and Inhibitors

Background: Fresh frozen plasma (FFP) is used to correct deficiencies of coagulation factors. The levels of these factors in FFP vary with donor demographics, ex-vivo processing of plasma. Quality control monitoring of FFP plays an important role.  

Aims: The aim of study was to analyze the levels of coagulation factors, the amount and structure of von Willebrand factor (VWF) in FFP units.

Methods: In total 30 FFP units were evaluated (20 from Foundation Pärnu Hospital Blood Centre and 10 from Ida-Viru Central Hospital Blood service). Blood of donors was collected with citrate, separated from blood erythrocytes, rapidly frozen within 24 hours after phlebotomy at –25°C. FFP was thawed at +37°C and immediately tested for fibrinogen, FII, FV, FVII, FVIII, FIX, FX, FXI, FXII, VWF:Ag levels, VWF activity (VWF:Ac) and VWF multimers (VWF:MM).

Results: All 30 FFP samples had normal coagulation factors activities, normal fibrinogen levels and the levels were within the routinely applied reference intervals. There was no significant difference in VWF:Ag, VWF:Ac and FVIII levels between blood group O (n=11) and non-O blood group (n=19) samples (p=0.182, p=0.271, p=0.401). The mean value of FVIII (after freezing and thawing) was not less than 70 IU of Factor VIII per 100 mL (mean 115 %, SD±35.7). The VWF:MM evaluation by densitometer demonstrated patterns comparable to internal reference plasma, which is used as internal quality control. No significant differences in high molecular weight multimers (HMWM) between blood group O and non-O samples were found (p=0.491).

Conclusions: Our results demonstrate that retention of all coagulation factors in FFP produced from whole blood within 24 hours of collection is satisfactory. The quantity and quality of VWF as well as VWF:MM patterns represent healthy population. To our best knowledge, this report on FFP quality is the first study in Estonia.

To cite this abstract in AMA style:

Pikta M, Szanto T, Reimal R, Muliin P, König H, Blinova G, Banys V. Analysis of Coagulation Factors in Fresh-frozen Plasma [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/analysis-of-coagulation-factors-in-fresh-frozen-plasma/. Accessed December 8, 2021.

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