Abstract Number: PB0550
Meeting: ISTH 2020 Congress
Background: Sickle cell disease (SCD) is a neglected genetic disease associated with high occurrence of thrombotic vaso-occlusive (VOC) crisis. In spite of exhaustive evidence of platelet’s hyperactivation in SCD, there is a lack of knowledge about the correlation between Megathrombocytes and the occurrence of VOC in SCD.
Aims: We aim to evaluate the correlation between platelet’s counts and the thrombotic state of patients using a new morphology-based evaluation method, which allows the determination of clinical indicators to predict VOC in a fast, easy and low-cost way.
Methods: Thirty-five patients diagnosed with SCD and receiving medical assistance at the University Hospital (UERJ, Brazil) participated in the study (ethics committee/ CEP-UERJ 455.394). D-dimer was quantified by ELISA to indicate the occurrence of thrombosis in patients. Platelets total count was obtained using the automatic counter. Blood smears were used for manually counting of platelets and megathrombocytes using a validated method developed by our group, called Modified Fonio’s method 2 (FM2), which allows the reading of 20 spots/ sample. All statistical analyses were performed by GraphPad Prism 5
Results: SCD patients treated and non-treated with Hydroxyurea (HU) have shown higher median platelets counts (PC) (>300×103/mm3, p< 0.01), D-dimer values (>300ng/ml, p< 0.05) and equal megathrombocytes counts (MC) (6,9%, p>0.05) when compared to healthy individuals. Platelets abnormalities such as higher granulations and megathrombocytes counts were found in non-treated group. FM2 analysis has shown a strong correlation between D-dimer levels and MC in patients without HU treatment (p< 0.05 and r=-0.45), different to FM method by Lima and co-works (2001).
Conclusions: Finally, our results demonstrated that FM2 analysis of megathrombocytes should be used as sign of occurrence of thrombotic VOC in SCD. As an easy, fast, and low-cost method, the FM2 can be a valuable tool to help prognosis assessment and treatment follow-up of SCD patients, especially in underdeveloped countries.
To cite this abstract in AMA style:Serrao LW, Silva Lisboa EFN, Rosa de Siqueira PF, Canellas da Silva MC, Medeiros J, Soares A, Fleury MK. Analysis of Megathrombocytes as a New, Fast, and Low Cost Tool to Indicate Thrombosis and Predict Prognosis in Sickle Cell Disease [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/analysis-of-megathrombocytes-as-a-new-fast-and-low-cost-tool-to-indicate-thrombosis-and-predict-prognosis-in-sickle-cell-disease/. Accessed November 26, 2020.
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