Background: : It is well recognized that blood platelets play crucial role in the pathologies linked to cardiovascular diseases (CVD). In this regard, the search for strong and safer antiplatelet agents is of high interest.

Aims: The aim of this study is to examine the anti-aggregant activity induced by collagen of butanolic extract of Rubia tinctorum L (BERT) in vitro and ex vivo.

Methods: by measuring the bleeding time and platelets count. Phytochemical analysis was assessed by measuring DPPH free radical-scavenging method. Identification and quantification of flavonoids compounds were determinate by HPLC analysis.

Results: Our finding demonstrated that B-RT extract showed a significant (P< 0.001) inhibition of collagen induced platelets aggregation (1.48 ± 0.76, n=3). These results correlate well with data of bleeding time and platelet count. Phytochemical analysis revealed that B-RT extract contain a high amount of total flavonoids 0.7± 0.055mg CAE/100g DM,HPLC revealed that the main polyphenols identified and quantified were vanillin (22.61%) in highest concentration followed by Rosmarinic acid (13.21%) and Cinnamic acid (13.04%) respectively, finally Quercetin (12.39%).

Conclusions: Rubia tinctorum butanolic extract exhibited a high antioxidant activity. The present investigation has shown that BERT extract has an anti-aggregant effect in which flavonoids could be involved, in particular vanillin.

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ISTH Congress Abstracts - https://abstracts.isth.org/abstract/anti-aggregant-effect-of-butanolic-extract-of-rubia-tinctorum-on-platelets-in-vitro-and-ex-vivo/