Abstract Number: PB0316
Meeting: ISTH 2021 Congress
Background: Annexin V is a glycoprotein with anticoagulant activity. IgG and IgM Anti-AnnexinV antibodies have the ability to displace annexin V from phospholipid surfaces and this has been linked with enhanced prothrombotic conditions such as vasoocclusive crisis, the hallmark of Sickle Cell Anaemia (SCA).
Aims: To determine the serum level of IgM and IgG antibodies to AnnexinV in SCA patients in crisis and steady states in order to assess the contribution of the procoagulant effect of Anti-AnnexinV to vasoocclusive crisis and its severity.
Methods: The study comprises of 90 SCA subjects; 35 subjects in vasoocclusive crisis (VOC), 35 subjects in steady states and 20 normal Haemoglobin (HbAA) control subjects. Anti-annexin V antibodies activity was determined with enzyme-linked immunosorbent assay (ELISA) KIT. Haematological parameters were analyzed using Haematology autoanalyser (SYSMEX).
Data analysis was done with the statistical package for social sciences version 21.
Results: The results showed that the prevalence of anti-annexinV IgG seropositivity was found to be 22.9% in crisis, 20.0% in steady state and 0.0% in controls. The seropositivity of IgM anti- annexin V was significantly higher in steady state than in control (p = 0.015), but in VOC the seropositivity of anti annexin V IgM though higher than in controls was not statistically significant (p=0.071).The mean platelet count in the study subjects were 287.8±125.1 x109cells/L, 288.4±132.6 x109cells/L and 179.5±56.4 x109cells/L, the difference in mean platelet count was significantly lower in the control subjects than in the SCA groups (p= 0.002).
Conclusions: Serum levels of anti-annexin V IgM and IgG do not significantly differ between steady state and VOC. There is positive and significant correlation between the platelet count and IgM anti-annexin V in patients with SCA in steady state.
To cite this abstract in AMA style:Edewor-Okiyo D, Adediran A, Ogbenna A, Olowoselu O, Omolade A, Idogun S. Anti-annexinv Serum Levels and Haematological Indices in Sickle Cell Anaemia Patients in Vasooclusive Crisis and Steady States Seen at Lagos University Teaching Hospital Lagos Nigeria [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/anti-annexinv-serum-levels-and-haematological-indices-in-sickle-cell-anaemia-patients-in-vasooclusive-crisis-and-steady-states-seen-at-lagos-university-teaching-hospital-lagos-nigeria/. Accessed September 22, 2023.
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