Abstract Number: PB0213
Meeting: ISTH 2020 Congress
Theme: Coagulation and Natural Anticoagulants » Coagulation Factors and Inhibitors
Background: The coagulation cascade is a dynamic physiological process that comprises a sequence of activation reactions for the formation of the blood cloth. This chain of reactions involves the interaction of numerous blood components known as coagulation factors, whose deregulation could generate hemorrhagic or thromboembolic events, such as venous thromboembolism (VTE), deep vein thrombosis (DVT) or pulmonary embolisms (PE).
The coagulation factor Xa (FXa), plays a central role in the convergence of intrinsic and extrinsic coagulation pathways; therefore, it is a critical factor for the research of novel anticoagulation strategies. Moreover, functional nanoparticles as emerged as an innovative therapeutical approach that allows improving the pharmacological properties of diverse types of drugs. In particular, polymeric nanoparticles lead the clinical translation of nanotechnology as drug delivery systems due to its recognized biodegradable and biocompatible properties.
Aims: In this work, we synthesized poly(lactide-co-glycolide) methyl ether-block-poly(ethylene glycol) (PLGA-PEG) nanoparticles functionalized with FXa piperazinone synthetic inhibitors developed in our laboratory.
Methods: Those molecules, as well as the polymeric coupling, were characterized by 1H-NMR. The size (200 nm) and zeta potential (surface charge, -30 mV) of the functional nanoparticles were analyzed by dynamic light scattering, the spherical morphology of nanoparticles using transmission electron microscopy.
Results: The inhibition capacity against FXa enzyme of functionalized nanoparticles was analyzed by SensoLyte ® Rh110 Factor Xa Assay Kit exhibited 70% of inhibition compared with the unbound molecules used as a control.
Conclusions: Our preliminary results strongly support the generation of a novel anticoagulant agent based on functional nanoparticles.
To cite this abstract in AMA style:
Salazar J, Rojas C, Santana-Romo F, Romero Bohorquez A, Vilos C, Zacconi F. Anticoagulant Effect of Novel Nanoparticles Functionalized with Synthetic Piperazinone Derivatives as FXa Inhibitors [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/anticoagulant-effect-of-novel-nanoparticles-functionalized-with-synthetic-piperazinone-derivatives-as-fxa-inhibitors/. Accessed September 29, 2023.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/anticoagulant-effect-of-novel-nanoparticles-functionalized-with-synthetic-piperazinone-derivatives-as-fxa-inhibitors/