Background: Immune thrombocytopenic purpura (ITP) is an autoimmune disorder. The exact triggering factor is still elusive. The peripheral destruction of platelets is result of various factors. One of the factor is oxidative stress which plays significant role not only damaging RBCs but also platelets.

Aims: The current study was planned to assess antioxidative capacity of platelets in the pathogenesis of ITP patients in Pakistan.

Methods: A case control study (1:1) was conducted from January 2015 to July 2017 with 111 ITP patients and 111 age matched controls. ITP patients were grouped as newly diagnosed/persistent ITP and chronic/refractory ITP patients. Peripheral blood was collected and CBC was recorded using XN 1000.The redox state of platelets was observed flow cytometrically in PRP using DCF and H2O2. The statistical analysis was determined by Kruskal Wallis,dunn’s test and Spearman correlations test using SPSS ver.23.

Results: A total of 111 ITP patients and healthy participants were included. The ITP patients were categorized as 38(34%) C-ITP, 32(29%) ND-ITP, 31(28%) P-ITP and 10(09%) R-ITP. The redox state of platelets was significantly deranged (p≤0.05) in all patients groups with raised MFI values in ND-ITP 462(213) followed by R-ITP 547(123), C-ITP 450(186), P-ITP 412(162) as compared to controls 389(188) revealing reduced AOC of platelets in ITP patients. There was significant (≤0.05) positive correlation between platelets and AOC in ND-ITP r=0.874**and C-ITP r=0.984**.

Conclusion(s): ITP is a condition with peripheral destruction of platelets with unknown specific triggering factor. AOC may also be included in early diagnostic model for better treatment option which will reduce drastic damage caused by OS.

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ISTH Congress Abstracts - https://abstracts.isth.org/abstract/antioxidative-capacity-of-platelets-in-the-pathogenesis-of-immune-thrombocytopenia-finding-the-forest-for-trees/