Abstract Number: PB1481
Meeting: ISTH 2020 Congress
Background: A combination of an ex vivo model for shear stress and easy-to-use laboratory method to determine platelet function in whole blood (WB) may be helpful to better understand the pathophysiology of both bleeding and thrombosis duringextracorporeal circulation (ECC).
Aims: To investigate platelet activation status in WB and platelet rich plasma (PRP) samples after shear stress simulation using ex vivo chandler-loop model.
Methods: Two polyvinyl chloride tubes coated with heparin were filled with citrated or heparinized WB or PRP samples from healthy donors. To stimulate shear stress tubes were rotated in a temperature-controlled water bath (99 rounds per minute, one hour, 37°C). Samples were obtained before and after rotation for flow cytometer analyses. They were incubated with buffer, ADP or TRAP and double-stained for CD41, CD62P and CD63.
Results: A reduction in alpha granule release was observed in citrated WB samples upon ADP and TRAP stimulation after ECC-simulation compared to steady-state samples (median FI of CD62P: after ADP stimulation 0.96 vs. 1.35, p=0.0006; and after TRAP stimulation 1.14 vs. 2.19, respectively, p=0.0006). Similar results were obtained with PRP samples (median FI in CD62P: after activation with ADP 0.96 vs. 1.22, p=0.0064; after activation with TRAP, 0.93 vs. 1.20, p=0.0175). Furthermore, significant decrease in delta-granule response to TRAP was observed in WB (median FI in CD63: after TRAP activation: 1.01 vs. 1.23, p=0.0262). Interestingly, significant reduction was observed in alpha granule release in heparinized blood samples obtained from chandler-loop compared to control samples (median FI in CD62P: after activation with ADP 1.05 vs. 1.45, p=0.0973; with TRAP 1.06 vs. 3.06, p=0.0064).
Conclusions: Our data confirm that increased shear stress conditions can cause dysfunction in delta as well as alpha granules. The usefulness of this new method to predict bleeding and thrombosis is under investigation in an ongoing clinical study of patients with heart assist devices.
To cite this abstract in AMA style:Hidiatov O, Marini I, Pelzl L, Rigoni F, Wagner M, Straub A, Bakchoul T. Assessment of Platelet Function after Shear Stress Simulation Using an ex vivo Model for Extracorporeal Circulation [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/assessment-of-platelet-function-after-shear-stress-simulation-using-an-ex-vivo-model-for-extracorporeal-circulation/. Accessed January 23, 2022.
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