Abstract Number: VPB0635
Meeting: ISTH 2022 Congress
Background: Atherothrombosis is one of the major risk factors associated with the increased incidence of cardiovascular diseases, particularly in developed countries. Natural-based products intervention has been suggested as one of the essential approaches for the management of atherothrombosis-related cardiovascular diseases (CVDs). Phytochemical compounds, including gingerol, hesperidin, naringin and citric acid have been reported to exhibit antioxidant, antihyperlipidemic, anti-inflammatory, antiplatelet, anticoagulant and antithrombotic properties.
Aims: The study determined the phytotherapeutic effects of herbal extracts; a combination of Zingiber officinale (ginger), Allium sativum (garlic), Citrus Limon (lemon), Malus Domestica (Apple)/ apple cider vinegar and Honey (ZACAH) on atherothrombosis-related markers in SD rats fed with HCD.
Methods: Identification of the major related phytochemical compounds present in ZACAH extracts was performed by ultra-high performance liquid chromatography-mass spectrophotometry (UHPLC-MS) profiling at both negative and positive models. Male SD rats were randomly divided into six groups, the normal diet (ND), HCD, treatment with simvastatin (TRTSM) at 10mg/kg of body weight, treatment with ZACAH extracts (TRT1) at 1mg, 3mg (TRT2), and 5mg (TRT3) per kilogram of body weight. At the end of the 2 weeks experiments, the animals were sacrificed and the activities of thrombosis-related proteins were studied.
Results: The UHPLC-MS profiling of the phytochemicals in ZACAH extracts yielded the identification of hesperidin (retention time (RT); 11.18), citric acid (2.18), gingerol (18.62) and naringin (10.07). Oral gavage supplementation with ZACAH extracts significantly attenuated the activities of plasminogen activator inhibitor-1 (PAI-1) and tissue factor (TF) while improving the levels of tissue-type plasminogen activator (t-PA) and nitric oxide (NO) in a dose-dependent manner.
Conclusion(s): The findings suggested that identified phytochemicals available in ZACAH extracts could inhibit the progress of atherothrombotic plaque development through suppressing the expression of thrombotic marker activities induced by dietary high cholesterol intake and may represent novel therapeutic opportunities for the treatment of atherothrombosis-related CVDs.
Figure1 : Expression of Atherothrombotic Related Proteins. Values are presented as mean ± SEM -n = 6-. Letter a indicates no significant difference between ZACAH extracts treatment groups, ND and TRTSM treatment groups, while letter b shows significant differences in the HCD group compared to ND, TRTSM and ZACAH extracts treatment groups -ANOVA, Tukey’s, p < 0.05-. NO; nitric oxide, TF; tissue factor, t-PA; tissue-type plasminogen activator, PAI-1; plasminogen activator inhibitor, ND; normal diet, HCD; high cholesterol diet, TRTSM; treatment with simvastatin at 10mg/kg of body weight, TRT1, 2 and 3; treatment with ZACAH extracts at 1,3 and 5mg/kg of body weight, respectively.
Figure 2 : The Standard Curve of each Atherothrombotic Related Protein and Coefficient of Determination -R2-. Data are expressed as mean ± SEM -n = 6-. NO; nitric oxide, TF; tissue factor, t-PA; tissue-type plasminogen activator, PAI-1; plasminogen activator inhibitor.
To cite this abstract in AMA style:KWAIFA I, Perumal K, Bahari H, Yong K, Eshak Z, Balan S, Abidin A, Ghadi F, Kong L, NOOR S. Attenuation of Thrombotic Biomarkers in a Hyperlipidaemic Sprague Dawley Rats Model [abstract]. https://abstracts.isth.org/abstract/attenuation-of-thrombotic-biomarkers-in-a-hyperlipidaemic-sprague-dawley-rats-model/. Accessed September 26, 2022.
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