Abstract Number: PO103
Meeting: ISTH 2021 Congress
Background: A plaque may rupture with high risk of subsequent thrombus mediated acute clinical events such as stroke. The efficacy of intravenous anti-anticoagulant drugs is limited owing to a relatively poor recanalization rate and incomplete function recovery in the majority of treated patients. Therefore, other treatment modalities should be explored.
Aims: The aim of this study was to generate a hamster model of common carotid artery atherothrombotic stenosis and the subsequent investigating the feasibility of combined catheter-based Q-Switched-Nd:YAG laser plus pulsed low level dual frequency focused ultrasound-mediated thrombolytic therapy in this model, wherein diagnostic B-mode ultrasound is combined with thrombolytic therapy system, with a goal of increased safety.
Methods: Briefly, golden Syrian hamsters were submitted to common carotid artery atherothrombotic stenosis by primary balloon injury followed 1.5% cholesterol- rich diet injury for 8 weeks and finally perivascular severe liquid nitrogen-based cold injury. Then treatment group underwent combined B-mode ultrasound-guided Q-switched Nd:YAG laser (λ= 532 nm, P= 10 W, PD= 5 ns) and pulsed low level dual frequency focused ultrasound (F= 750 KHz, I= 10W/cm2, Duty Factor= 20% and F= 1.2 MHz, I= 24W/ cm2, Duty Factor= 50%)- mediated thrombolytic therapy.
Results: Results from histopathology, B-mode and color Doppler ultrasonography, showed a significant reduction in the mean value for thrombus content, percentage of luminal cross-sectional area of stenosis, blood mean velocity and a significant increase in the mean value for blood volume flow at the stenotic region in the treatment group compared with the other groups (P<0.05).
Conclusions: Enhanced anti- thrombotic effect of mechanical inertial cavitation bubbles, induced by combined Q- Switched- Nd:YAG laser and pulsed low level dual frequency focused ultrasound, can cause to reduce the thrombus content and significantly dilate the luminal cross-sectional area of stenosis and lower treatment time.
To cite this abstract in AMA style:Mehrad H, Farhoudi M. B-mode Ultrasound-guided Combined Catheter-based Q-switched-Nd: YAG Laser and Pulsed Low Level Dual Frequency Focused Ultrasound Thrombolytic Therapy Reduce Thrombus Content in the Arterial Atherothrombotic Stenosis [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/b-mode-ultrasound-guided-combined-catheter-based-q-switched-nd-yag-laser-and-pulsed-low-level-dual-frequency-focused-ultrasound-thrombolytic-therapy-reduce-thrombus-content-in-the-arterial-atherothro/. Accessed September 29, 2023.
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