Abstract Number: PB1291
Meeting: ISTH 2022 Congress
Theme: Vascular Biology » Inflammation and Sepsis
Background: HIV-infected patients are at increased risk of vascular disease and arterial thrombosis, linked to endothelial dysfunction and chronic inflammation.
Aims: To evaluate the presence of biomarkers of endothelial dysfunction in plasma the coagulation status of leukocytes in HIV-infected patients.
Methods: Venous blood was collected with approval from the Wits Human Research Ethics Committee from healthy controls (n=13) and compared with samples collected from HIV-infected patients (n=22). Biomarker levels in plasma were evaluated using the MILLIPLEX MAP Human Cardiovascular Disease (CVD) Magnetic Bead Panel 2 (Merck KGaA, Darmstadt, Germany). Flow cytometry was performed on an LSR II flow cytometer and analysed on FlowJo (both Becton-Dickinson, Franklin Lakes, USA). Monocytes and neutrophils were identified by light scatter characteristics and expression of HLA-DR, CD33, CD14, CD16 and CD69 (monocytes) and CD33, CD16 and CD69 (neutrophils). Tissue factor was expressed as a percentage of positive cells. Descriptive statistics were computed and values were compared with a Mann-Whitney U test on Graphpad Prism (Graphpad, California, United States). A p-value of < 0.05 was considered significant.
Results: HIV-infected patients showed elevated humoral markers of inflammation and endothelial dysfunction (GDF-15, p-selectin and sICAM-1) when compared with uninfected controls. The leukocytes in these patients showed upregulation of the procoagulant tissue factor expression (Table 1).
Conclusion(s): HIV infection predisposes to endothelial dysfunction. In this study, we investigated the contribution of both humoral and cellular factors to increased cardiovascular risk. GDF-15, an independent biomarker of cardiovascular disease and sICAM-1 and p-selectin, markers associated with leukocyte trafficking across the endothelium were significantly elevated in HIV-infected patients in the absence of clinical cardiovascular disease. Tissue factor expression by leukocytes was also increased and which contributed to a general procoagulant state. Future directions will examine these factors in HIV-infected patients with established vascular disease.
Table
Table 1: Expression of Tissue factor and biomarkers of endothelial disease in HIV-infected patients compared with uninfected controls
To cite this abstract in AMA style:
Mayne E, Abdool-Carim T, Lederman M, Funderburg N, Louw S. Biomarkers of endothelial cell dysfunction in HIV-infected patients [abstract]. https://abstracts.isth.org/abstract/biomarkers-of-endothelial-cell-dysfunction-in-hiv-infected-patients/. Accessed October 1, 2023.« Back to ISTH 2022 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/biomarkers-of-endothelial-cell-dysfunction-in-hiv-infected-patients/