Abstract Number: PB0610
Meeting: ISTH 2020 Congress
Background: Bone marrow (BM) cell populations have traditionally been assessed visually by light microscopy because most automated analyzers cannot adequately capture immature cells; however, advances in modern hematology analyzers have improved detection of immature cells. The rapid availability of complete blood count (CBC) results can be advantageous for patients and clinicians, but comprehensive CBC data using the new analyzer methods for BM samples are lacking.
Aims: The aim was to i) identify and count BM cells, ii) generate a gating algorithm for cells isolated from BM aspirates using a novel hematology analyzer.
Methods: Two hundred and sixteen BM aspirates were collected from 186 patients with oncohematologic diseases (leukemia n:68, multiple myeloma n:55, lymphoma n:22, myelodysplastic syndrome n:17, myelofibrosis n:15, aplastic anemia n:9) and 30 healthy donors for allogeneic transplantation. All samples were analyzed on a modified Sysmex XN20 Module® (Sysmex, Kobe, Japan), and were also blindly assessed by a hematology expert under microscopy. Data from the Sysmex XN20 Module® were saved as .fcs files and then manually gated using the Software version 2.5.1 (Perttu Terho, Turku, Finland). Total nucleated cell counts were recorded and used to determine cellularity, myeloid/erythroid (M/E) ratio, and granulocyte/erythrocyte (G/E) ratio. Results were analyzed statistically.
Results: Compared with the healthy donors’ findings, degree of cellularity was significantly higher for the patients with leukemia and lymphoma, respectively, and was significantly lower for patients with aplastic anemia (p< 0.05 for all). As well, mean M/E ratio was significantly higher for patients with leukemia and lymphoma, respectively, and mean G/E ratio was significantly higher for patients with leukemia (p< 0.05 for all). Three parameters were significantly correlated between the Sysmex XN20 Module®-analyzed and microscopy-evaluated BM specimens: cellularity r=0.18, p=0.02; M/E ratio r=0.76, p=0.01; G/E ratio r=0.78, p=0.001.
Conclusions: Our preliminary findings warrant further investigation to identify clinically useful novel analyzer parameters for BM cells.
To cite this abstract in AMA style:
Kaya Z, Kirkiz S, Aydin Kaynar L, Özkurt ZN, Koçak Ü, Yağci M. Can Bone Marrow Cells Be Differentiated and Counted Using a Novel Hematology Analyzer? [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/can-bone-marrow-cells-be-differentiated-and-counted-using-a-novel-hematology-analyzer/. Accessed March 21, 2024.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/can-bone-marrow-cells-be-differentiated-and-counted-using-a-novel-hematology-analyzer/