Abstract Number: PB0437
Meeting: ISTH 2020 Congress
Background: Circulating microRNAs and extracellular vesicles (EVs) were found to be modulated in stroke.
Aims: We identified all miRNAs as well as determined EVs from platelets (PEVs), leukocytes (LEVs) and endothelial cells (EEVs) as biomarkers to diagnose stroke.
Methods: Blood collection at days 1 / 7 after the onset of stroke patients, and controls. Plasma EVs: Flow cytometry (Apogee A60); pletelets (CD61, CD62p; PEVs), leukocytes (CD45; LEVs) and endothelial cells (CD146; EEVs). Plasma RNA extraction: mirVANA Kit and quality assesment: Agilent 4200 TapeStation. MiRNA profiling: Affymetrix GeneChip 4.0.
Results: Patient with stroke had higher platelet reactivity comparison to controls (ADP p< 0.001, TRAP p< 0.001). Most down-regulated; miR-3135b (p< 0.001), associated with vascular calcifications/HF; miR-1275 (p=0.028), involved in atherosclerosis; miR-4467 (p=0.003), and miR-7170 (p< 0.001) modulated in neurodegenerative diseases. Most up-regulated; miR-18a (p=0.004), associated with stroke/FraminghamCohort; the platelet-enriched miR-22-5p (p=0.004), modulated in Huntington Disease; miR-199a (p=0.012), marker of brain microvascular injury/stroke severity, miR-106b (p=0.009), regulator of neural stem-cell proliferation/differentiation was found (figure1). PEV concentrations were elevated, both at day 1 / 7 (p=0.035, p=0.059, respectively). LEVs were comparable at day 1 (p=0.83) and decreased at day 7 (p=0.059), whereas EEVs decreased at day 1 (p=0.048) and normalized to control levels at day 7 (p=0.91) (figure2).
Conclusions: Several miRNAs were identified that are known to play a role in the pathophysiology of neurovascular diseases, paving the way to a new class of smart pathophysiology-based biomarkers in stroke. PEVs concentrations are elevated in patients with stroke both at day 1 and day 7, compared to controls. LEVs and EEVs are fluctuate between day 1 and day 7 after stroke, likely reflecting activation of immune system and endothelium following brain damage. Study was supported by Polish Science Center grant ´´Preludium´´ number 2017/25/N/NZ5/00545.
To cite this abstract in AMA style:Eyileten C, Czajka P, Jakubik D, Jarosz-Popek J, Fitas Z, Nowak A, Zareba L, von Baumgarten G, De Rosa S, Gasecka A, Wolska M, Sharif L, Mirowska-Guzel D, Czlonkowska A, Postula M. Circulating MicroRNAs and Platelet Extracellular Vesicles as Novel Biomarkers in Acute Ischaemic Stroke [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/circulating-micrornas-and-platelet-extracellular-vesicles-as-novel-biomarkers-in-acute-ischaemic-stroke/. Accessed January 23, 2022.
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