Abstract Number: PB0563
Meeting: ISTH 2020 Congress
Background: The evaluation of platelet function by aggregometry techniques is complex, time consuming, with a high manual component that requires dedicated personel and expert teams. The Sysmex CS-2500 (Siemens) is a high performance automated coagulation analyzer designed for aggregometry studies.
Aims: To assess the efficacy, reliability and ability to detect alterations of platelet function in the Sysmex CS-2500 in a comparative study with conventional aggregometry (APACT, Helena), both using light transmission aggregometry (LTA).
Methods: We obtained platelet rich plasma from healthy individuals, from patients with suspected platelet functional disorders and from blood samples exposed to antiplatelet therapy. We evaluated the aggregation response against arachidonic acid (1.4 mM), ADP (4 µM), collagen (2.5 g / ml), epinephrine (10 µM) and ristocetin (1 mg / ml) in 48 samples. We analyzed the correlation between the results with both techniques and used the Bland-Altman test to calculate the confidence intervals of the differences.
Results: The results obtained with the two techniques showed a good correlation (r = 0.79; p < 0.001). The degree of coincidence between the results in the two aggregometers was very high, with 95% of the results included within the standard deviations of the mean (image). The individual results of the different aggregating agents was similar in the two types of aggregometer. The Sysmex CS-2500 detected the presence of anomalies n a similar way to the APACT used as a reference. No technical difficulties were observed in the management of the automated equipment.
Conclusions: LTA is still considered the “gold standard” technique for the evaluation of platelet function. The CS-2500 offers diagnostic possibilities similar to conventional aggregometry in a high performance desktop coagulometer. This automated equipment may offer alternatives in the future allowing laboratories to assess and diagnose the different alterations of primary and secondary hemostasis.
[Bland-Altman plot]
To cite this abstract in AMA style:
Ramos A, Pino M, Molina P, Moreno Castaño AB, Díaz-Ricart M, Escolar G. Comparative Evaluation of the Aggregation Studies in a Sysmex CS2500 Coagulometer with a Standard Turbidimetric Technique [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/comparative-evaluation-of-the-aggregation-studies-in-a-sysmex-cs2500-coagulometer-with-a-standard-turbidimetric-technique/. Accessed October 2, 2023.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/comparative-evaluation-of-the-aggregation-studies-in-a-sysmex-cs2500-coagulometer-with-a-standard-turbidimetric-technique/