Abstract Number: PB0543
Meeting: ISTH 2020 Congress
Background: The Sysmex CN-6000 analyzer utilizes light transmission aggregometry (LTA), the current gold standard method for assessing platelet function. The new parameters; adenosine diphosphate (ADP)-induced PAL (APAL) and collagen-induced PAL (CPAL) have been developed to aid with interpretation of LTA results. The APAL and CPAL parameters are scored from 0 to 10 and are able to be calculated based on platelet aggregation patterns upon exposure to 1 and 10 µM ADP and 1 and 5µg/mL Collagen. The CN-6000 has been developed to perform automated reagent/agonist dilution for platelet aggregation testing.
Aims: The aim of the study was to evaluate performance of platelet aggregation function of the new fully automated Sysmex coagulation analyzer CN-6000.
Methods: A comparative study between the CN-6000 and CS-5100 (SYSMEX) was performed using 85 platelet rich plasma (PRP) samples for ADP testing and 82 PRP samples for Collagen testing at different levels of aggregation by adding several concentration of the anti-platelet drug, Cangrelor (P2Y12 inhibitor) or acetylsalicylic acid (Aspirin) to healthy volunteer PRP samples.
Results: Excellent correlations were observed between CN-6000 and CS-5100. For maximal aggregation, y = 1.00x +3.80, r = 0.987 and y = 0.89x + 11.48, r = 0.957 for 1 and 10 µM ADP and y = 1.02x – 0.89, r = 0.996 and y = 0.99x + 0.65, r = 0.973 for 1 and 5µg/mL Collagen, respectively. For new scoring system, y = 0.91x + 0.94, r = 0.971 and y = 1.00x – 0.08, r = 0.994 for APAL and CPAL, respectively.
Conclusions: Our results demonstrated that the CN-6000 is a robust automated coagulation analyzer for platelet aggregation testing including the new APAL and CPAL scoring system. The CN-6000 also provides high analytical performance as a high throughput analyzer for routine and specific coagulation parameters and is suitable for total laboratory automation.
To cite this abstract in AMA style:Egashira M, Kitano K, Narisada R, Kono M, Nakajima K, Sakayori T. Comparison of New Scoring System of Platelet Aggregation on Automated Coagulation Analyzer CN-6000 with CS-5100 [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/comparison-of-new-scoring-system-of-platelet-aggregation-on-automated-coagulation-analyzer-cn-6000-with-cs-5100/. Accessed October 2, 2023.
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