Abstract Number: PB1364
Meeting: ISTH 2020 Congress
Background: Immune thrombocytopenia (ITP) is a disease caused by the production of anti-platelet autoantibodies that destroys platelets. The basic method assessing PLT is peripheral blood morphology with EDTA or with citrate. The older is counting PLT under the microscope (FONIO), newer and more accurate is fluorescence method. The method still difficult to access but raising great hopes is the evaluation of PLT by flow cytometry.
Aims: The purpose of this study is to compare PLT using the various avaliable diagnostic methods.
Methods: PLT were assessed by five methods in adult patients with ITP (peripheral blood with EDTA and citrate, FONIO, fluorescence and immunofluorescence).
Results: 66 patients with newly diagnosed, previously untreated ITP were enrolled in the study. The median age at diagnosis was 56 years (+/-19) and 56% were men. The mean PLT in the EDTA method was 74 G/l (SD +/-43), with citrate 73 G/l (SD +/-46), fluorescent 76 G/l (SD +/-44), FONIO 103 G/l (SD +/-64), immunofluorescent 97 G/l (SD +/-65). EDTA method was compared with FONIO, citrate, fluorescent and immunofluorescence. The significant difference in PLT was observed in the results comparing the immunofluorescence method (53% +/-123%), followed by the FONIO method (51% +/- 91%). No difference was observed when comparing the standard method with the fluorescence method and citrate morphology. PLT results obtained from the immunofluorescence method differed from the fluorescent method by 40% +/-78%. The obtained results were correlated with standard morphological platelet parameters (PDW, MPV, P-LCR), white and red blood cell counts, immature platelet fraction and mean fluorescence intensity.
Conclusions: Based on the results, the most reflective method of PLT is the immunofluorescence. Unfortunately, this expensive and labor-intensive method, should be reserved for selected patients. For more accurate knowledge larger group with various types of thrombocytopenia should be evaluated.
To cite this abstract in AMA style:Witkowski M, Witkowska M, Tybura-Sawicka M, Majchrzak A, Treliński J, Chojnowski K, Robak T, Smolewski P. Comparison of Various Diagnostic Methods in Assessing Platelet Count in Patients with Immunotrombocytopenia [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/comparison-of-various-diagnostic-methods-in-assessing-platelet-count-in-patients-with-immunotrombocytopenia/. Accessed January 23, 2022.
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