Abstract Number: PB1406
Meeting: ISTH 2020 Congress
Background: High on‐treatment platelet reactivity is a potential risk factor for adverse cardiac events in patients taking anti-P2Y12 drugs.
Aims: Correlation of VASP phosphorylation with light transmission aggregometry assays.
Methods: Whole blood samples from 20 individuals with no reported hemostasis disease and 21 patients treated with aspirin and either clopidogrel (n=11) or ticagrelor (n=10) were tested head-to-head by the consensus recommended platelet function tests (PFT) including vasodilator‐stimulated phosphoprotein phosphorylation (VASP) and light transmission aggregometry (LTA) upon stimulation with 10 and 20 µM of adenosine diphosphate (ADP). All tests were performed using standardized procedures within 3 hours after sampling. Correlation between assays was tested using Microsoft Excel.
Results: The correlation between the VASP assay and LTA at 10µM ADP was high for samples from clopidogrel treated-patients (0.7-0.8), moderate for ticagrelor (0.5-0.6) and low for controls (< 0.2). When combining clopidogrel and ticagrelor samples together, the correlation raised above 0.8. For a 20µM ADP stimulation, the correlation between the VASP assay and LTA was >0.8 for clopidogrel samples; > 0.5 for ticagrelor and < 0.1 for control samples. Similarly, the correlation was found > 0.8 for samples from clopidogrel- and ticagrelor-treated patients.
Conclusions: Despite that the VASP assay and LTA upon 10 or 20µM ADP stimulation are correlated for patients treated with either clopidogrel or ticagrelor, the agreement among tests is higher (> 0.8) considering results obtained from both clopidogrel and ticagrelor altogether.
To cite this abstract in AMA style:Pons C, Moulard M, Canault M, Ibrahim M, Alessi M-. Correlation of VASP Phosphorylation Assay with Light Transmission Aggregometry for Patients Treated with Clopidogrel or Ticagrelor [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/correlation-of-vasp-phosphorylation-assay-with-light-transmission-aggregometry-for-patients-treated-with-clopidogrel-or-ticagrelor/. Accessed October 1, 2023.
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