Abstract Number: VPB0350
Meeting: ISTH 2022 Congress
Theme: Platelet Disorders, von Willebrand Disease and Thrombotic Microangiopathies » Platelet Antagonists and Novel Therapeutics
Background: Curcumin is a natural polyphenol derived from turmeric plant (Curcuma longa) that has many beneficial effects including inhibition of platelet activation and thrombus formation. Previously, we showed that curcumin by activation of adenosine A2A receptor stimulates AC/cAMP/PKA inhibitory pathway in platelets. We hypothesized that curcumin could potentiate anti-thrombotic effect of P2Y12 receptor inhibitor.
Aims: The aim of this study was to elucidate whether curcumin could potentiate the inhibitory effect of cangrelor (AR-C69931) on platelet aggregation induced by ADP.
Methods: Washed platelets from healthy volunteers were incubated with curcumin and aggregation was initiated by ADP. Laser diffraction method was applied for analysis of platelet aggregation. Activation of AC/cAMP/PKA inhibitory system of platelets was analyzed by Western blotting.
Results: Curcumin alone inhibited 1 µM ADP-induced platelet aggregation started from 50 µM which correspond to strong VASP phosphorylation and formation of procoagulant platelets. However, low curcumin concentrations (5-10 µM) did not significantly inhibit platelet aggregation and only slightly increased VASP phosphorylation. Preincubation with AR-C69931 (1 nM) prevented (54± 14%) ADP-induced aggregation. Combination of curcumin (5 µM) with AR-C69931 (1 nM) inhibited aggregation up to 86±4.9%. Both substances alone only slightly increased VASP phosphorylation, whereas their combination induced strong phosphorylation of VASP which correlate with a strong inhibition of aggregation.
Conclusion(s): In summary, we showed that curcumin at low concentration potentiated inhibitory effect of cangrelor in ADP-stimulated platelet aggregation and VASP phosphorylation. Curcumin alone or in combination with other drugs is often used in clinical practice and presented data illustrated that low concentrations of curcumin, in combination with P2Y12 receptor antagonists, could be used as supplementation to prevent platelet activation.
To cite this abstract in AMA style:
Rukoyatkina N, Shpakova V, Gambaryan S. Curcumin potentiates an inhibitory effect of cangrelor on platelets [abstract]. https://abstracts.isth.org/abstract/curcumin-potentiates-an-inhibitory-effect-of-cangrelor-on-platelets/. Accessed October 1, 2023.« Back to ISTH 2022 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/curcumin-potentiates-an-inhibitory-effect-of-cangrelor-on-platelets/