Abstract Number: PB2147
Meeting: ISTH 2020 Congress
Theme: Venous Thromboembolism and Cardioembolism » Cancer Associated Thrombosis
Background: Inflammation and other vascular factors contribute to the pathogenesis of thrombotic complications in lymphoma patients. Biomarkers of hemostatic activation, vascular dysfunction and inflammation are upregulated in lymphoma.
Aims: This study was designed to compare the thrombin generation potential and its relevance to the generation of various biomarkers of hemostatic activation process and inflammatory responses.
Methods: Citrated blood samples from 90 patients with confirmed diagnosis of lymphoma were collected from University of Belgrade, Serbia. 50 samples of normal human plasma (NHP) were included as control. Thrombin generation studies were carried out using a commercially available kinetic system. Thrombin generation parameters such as peak thrombin (PT), lag time (LT) and area under the curve (AUC) were compiled. Plasma levels of D-Dimer, CRP, vWF and TNF-α were measured by using commercially available Sandwich ELISA assays.
Results: On a cumulative basis, lymphoma patients showed increase in LT (2.91.17) compared to NHP (2.7). The PT levels (119.046.2) and AUC was decreased (670.2290.0) in the lymphoma patients compared to NHP (PT: 159.731.1 and AUC: 700.9). When the lymphoma patients were sub-grouped, PT levels were similar in HL, NHL and CLL and were decreased compared to NHP. However, the AUC was increased in HL, decreased in NHL and was comparable for CLL compared to NHP. The lymphoma patients also exhibited elevated levels of D-Dimer CRP, vWF and TNF-α compared to NHP. In HL group D-Dimer and vWF correlated well with PT, in this group CRP, vWF and TNF-α also showed a good correlation with AUC. In the NHL group, CRP correlated well with LT.
Conclusions: The observed decrease in thrombin generation potential in these patients may be due to the consumption of prothrombin and other coagulation factors. Wide variations in circulating levels of various biomarkers and thrombin generation parameters between various lymphoma groups are noted.
| Samples | D-Dimer (ng/ml) AUC | D-Dimer (ng/ml) PT | CRP (ug/ml) AUC | CRP (ug/ml) PT | vWF (%) AUC | vWF (%) PT | TNF-α (pg/ml) AUC | TNF-α (pg/ml) PT |
| HL (n=7) | 0.7275 | 0.4664 | 0.5266 | 0.4988 | 0.3290 | 0.4813 | 0.2794 | 0.1825 |
| NHL (n=61) | 0.0149 | 0.0137 | 0.0104 | 0.0004 | 0.0083 | 0.0004 | 0.0509 | 0.0294 |
| CLL (n=22) | 0.0228 | 0.0027 | 0.0792 | 0.0064 | 0.0068 | 0.0267 | 0.1373 | 0.0889 |
| Lymphomas | 0.0362 | 0.0311 | 0.0401 | 0.0070 | 0.0123 | 0.0125 | 0.0440 | 0.0220 |
[Correlation coefficient demonstrating the relevance of various biomarkers to thrombin generation parameters]
To cite this abstract in AMA style:
Siddiqui F, Bontekoe E, Antic D, Hoppensteadt D, Gerotziafas G, Fareed J. Decreased Thrombin Generation Potential in Lymphoma Patients Is Associated with Increased D-dimer, CRP, vWF and TNF-α. Interrelationship between Thrombogenesis and Inflammation [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/decreased-thrombin-generation-potential-in-lymphoma-patients-is-associated-with-increased-d-dimer-crp-vwf-and-tnf-%ce%b1-interrelationship-between-thrombogenesis-and-inflammation/. Accessed September 22, 2023.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/decreased-thrombin-generation-potential-in-lymphoma-patients-is-associated-with-increased-d-dimer-crp-vwf-and-tnf-%ce%b1-interrelationship-between-thrombogenesis-and-inflammation/