Abstract Number: PB2014
Meeting: ISTH 2020 Congress
Background: Neutrophil extracellular traps (NETs) are formed when activated neutrophils secrete decondensed intracellular DNA together with antimicrobial enzymes such as myeloperoxidase (MPO). Since NETs are involved in the development of thrombosis, it is relevant to know which demographic and clinical characteristics are associated with the formation of NETs.
Aims: To determine the demographic and clinical characteristics that are associated with NET formation in the general population.
Methods: Citrated plasma was collected from 6449 participants (aged ≥55 years) included in the prospective population-based Rotterdam Study. Levels of NET formation were quantified by measuring levels of MPO-DNA complexes using an enzyme-linked immunosorbent assay. We used linear regression to determine the associations of MPO-DNA complex with age, sex, cardio-metabolic risk factors (e.g. hypertension, cholesterol, diabetes), a history of cardiovascular disease, and blood markers of inflammation and coagulation.
Results: The median age of the participants was 68.6 years (Interquartile Range (IQR) 62.8-75.3 years) and 56.3% of the participants were female. Median MPO-DNA complex levels were 0.05 AU/mL (IQR 0.04-0.09). We found an inverse association between MPO-DNA complex levels and the following characteristics: age (β -0.04 AU/mL/10 years, 95% confidence interval (CI) -0.06;-0.02), a history of coronary heart disease (CHD) (β -0.10 AU/mL, 95% CI -0.17;-0.03), the use of lipid-lowering drugs (β -0.06 AU/mL, 95% CI -0.12;-0.01), and levels of HDL-cholesterol (β -0.07 AU/mL/ mmol/L, 95% CI -0.12;-0.03). MPO-DNA complex levels were not related to sex, other cardio-metabolic risk factors, and markers of inflammation and coagulation.
Conclusions: Our results suggest that increasing age, a history of CHD, the use of lipid-lowering drugs and higher HDL-cholesterol levels are associated with lower levels of MPO-DNA complexes, a marker of NET formation. These results can lead to a better understanding of individuals who are at risk of developing NET-associated thrombotic diseases.
To cite this abstract in AMA style:Donkel SJ, Wolters FJ, Ikram MA, de Maat MPM. Determinants of Neutrophil Extracellular Trap Formation in the General Population: Based on the Rotterdam Study Cohort [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/determinants-of-neutrophil-extracellular-trap-formation-in-the-general-population-based-on-the-rotterdam-study-cohort/. Accessed November 26, 2020.
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