Abstract Number: PB0129
Meeting: ISTH 2021 Congress
Background: Circulating DNA or Neutrophil extracellular traps (NETs) modify fibrin structure (Longstaff C et al. The journal of biological chemistry 2013).
Aims: Interpretation of the results of plasma DNA and Histones, in peritoneal cancer patients.
Methods: 77 patients with peritoneal carcinomatosis of several origins (ovarian19, colon 22, pseudomyxoma 9, gastric 9, appendix 4, others origins 13) were tested. Nets in plasma were evaluated by measuring DNA by fluorimetry using Green fluorescence protein, Histone 3 and Histone 4 were determined by ELISA.
Results: i) The levels of circulating DNA and of Histone-3 in the 77 plasmas of cancer patients were found to be higher than in controls (mean levels of DNA and of H3 were respectively 0,84+/-0,97 and 2,71+/-4,28 in cancer patients versus 0,27+/-0,07 and 0,56 +/-0,06 in controls),
ii) DNA and H3 as well as H4 levels varied according to the origin of cancer: Patients with gastric cancer, pseudomyxoma and cancer of appendix expressed respectively 8, 5 and 8 times more Histone-3 than controls,
iii) 40 patients with cancer (about 50%) have an increased level of DNA but only 20 of them presented an increased level of Histone 3
iv) Of the 37 other patients for whom plasma level of DNA was normal, 11 presented increased levels of Histone H3,
v) The levels of Histone 4 were found to be higher in patients mainly with gastric cancer and pseudomyxoma (2.4 and 3.4 times more respectively) than in controls.
Consequently, the increased levels of DNA and of histone H3 in the 51 patients of the 77 with cancer testify an activation of polynuclear cells. Therefore, it was concluded that 66 % of cancer patients present leukocyte activation.
Conclusions: Circulating DNA and Histone-3 were increased mainly in plasma from patients with gastric cancer, pseudomyxoma and cancer of appendix, and are associated with leukocyte activation in these patients.
To cite this abstract in AMA style:Mirshahi S, Soria J, Aldybiat I, Pocard M, Mirshahi M. Determination of DNA and Histone 3 Plasma Levels for Evaluation of Leukocyte Activation in Plasma of Patients with Peritoneal Carcinomatosis [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 1). https://abstracts.isth.org/abstract/determination-of-dna-and-histone-3-plasma-levels-for-evaluation-of-leukocyte-activation-in-plasma-of-patients-with-peritoneal-carcinomatosis/. Accessed September 24, 2021.
« Back to ISTH 2021 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/determination-of-dna-and-histone-3-plasma-levels-for-evaluation-of-leukocyte-activation-in-plasma-of-patients-with-peritoneal-carcinomatosis/