Abstract Number: PB0122
Meeting: ISTH 2020 Congress
Background: A plaque may rupture with high risk of subsequent thrombus- mediated acute clinical events such as myocardial infarction and stroke.
Aims: The aim of this study was to generate a reproducible experimental rabbit femoral artery model of atherothrombotic stenosis with morphological similarities to the human disease.
Methods: Briefly, New Zealand white rabbits were submitted to atherothrombotic stenosis by primary perivascular monopolar high- frequency electrical current (Iavg= 150 mA, F= 250 KHz)- mediated endothelial injury followed by a 1.5% cholesterol- rich diet injury and finally perivascular severe cold injury using liquid nitrogen (-196 0C) at the femoral artery.
Results: Histopathology results showed the formation of advanced atherosclerosis with lipid-laden cells, atheroma, neovessel- rich plaque and severe stenosis (> 70%) in all of the rabbits´ arteries after 12 weeks. Four weeks after perivascular severe cold injury, results from histopathology and ultrasonography showed the formation of atherothrombotic stenosis in all of the rabbits´ arteries. Results from color Doppler ultrasonography at the stenotic region showed a significant increase in the mean value for blood mean velocity, wall mean thickness, percentage of luminal cross- sectional area of stenosis and formation of intraplaque hemorrhage and significant reduction in the mean value for blood volume flow in the atherothrombotic group compared with the control group
(p < 0.05).
Conclusions: In conclusion, we successfully produced thrombosis on ruptured atherosclerotic plaque in the rabbits´ arteries, that is similar to the condition seen in patients. This condition in rabbits can be properly assessed by color Doppler ultrasonography and histopathology.
To cite this abstract in AMA style:Mehrad H, Farhoudi M. Developing of a New Animal Artery Model of Atherothrombotic Stenosis using Perivascular Monopolar High-Frequency Electrical Current-Mediated Endothelial Injury Followed by a Cholesterol-Rich Diet Injury and Finally Perivascular Severe Cold Injury using Liquid Nitrogen [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/developing-of-a-new-animal-artery-model-of-atherothrombotic-stenosis-using-perivascular-monopolar-high-frequency-electrical-current-mediated-endothelial-injury-followed-by-a-cholesterol-rich-diet-inju/. Accessed May 6, 2021.
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