Abstract Number: PO109
Meeting: ISTH 2021 Congress
Background: The use of animal models in ischemic stroke has improved our understanding of stroke pathophysiology. An ideal animal model for atherosclerosis-mediated stroke would mimic the pathophysiology in humans.
Aims: We sought to develop an easily reproducible and inexpensive experimental hamster carotid artery model to study severe stenosis (>80%) with thick-cap fibroatheromatic plaque.
Methods: Thick- cap fibroatheromatic plaque was induced via perivascularly using high power blue laser (λ= 450 nm, P= 20 W) thermal injury at the right common carotid artery in Golden Syrian Hamsters. Hamsters were then fed a 2% cholesterol-rich diet for six weeks. Histopathology was evaluated after six weeks.
Results: Histopathologic evaluation revealed progressive smooth muscle cell and extracellular lipid droplet proliferation in the intimal layer, resulting in vessel wall thickening, increased inflammation and a large immature lipid core formation with a thick- fibrous cap. In addition, increased collagen-derived extracellular matrix was found in the intimal layer compared with the other groups. These conditions resulted in the formation of a thick-cap fibroatheromatic plaque. Percentage of luminal-cross sectional area of stenosis was 81.42[ASQ1] ± 6.31 (P < 0.05) compared with the other groups. Results from color Doppler ultrasonography showed a significant increase in the mean value for blood Peak Systolic Velocity (PSV), End Diastolic Velocity (EDV), Mean Velocity (MV), Resistance Index (RI), Pulsatility Index (PI) and significant reduction in the mean value for blood Volume Flow (VF) at the stenotic region in the atherosclerotic group compared with the control group (p < 0.05).
Conclusions: We successfully produced an easily reproducible and inexpensive [ASQ1] experimental hamster cerebrovascular model of atherosclerotic thick-cap plaque using high power blue laser- mediated thermal injury, similar to the condition seen in human subjects. This condition in hamsters can be properly assessed by color Doppler ultrasonography.
To cite this abstract in AMA style:Mehrad H, Sultan- Qurraie A. Development of an Experimental Animal Carotid Artery Model of Thick-cap Fibroatheromatic Plaque with Severe Stenosis (>80%) Using High Power Blue Laser-mediated Thermal Injury and High-cholesterol Diet Injury [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 1). https://abstracts.isth.org/abstract/development-of-an-experimental-animal-carotid-artery-model-of-thick-cap-fibroatheromatic-plaque-with-severe-stenosis-80-using-high-power-blue-laser-mediated-thermal-injury-and-high-cholesterol-d/. Accessed September 24, 2021.
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