Background: Disturbance in red blood cell (RBC) gas transport function can lead to hypoxic state and it is significant to detect in pregnant state. In the early stages of pregnancy, hypoxia causes the appearance of embryo pathophysiology. It seems necessary to develop diagnostic system for prediction the risk of hypoxia by detailed characterization of morphological and functional properties of red blood cells. It consist of shape parameters, hemoglobin content, the rate of anion permeability (active state of Band 3 proteins) and critical membrane tension.

Aims: To establish the essential deviations in RBC gas transport functions in healthy and pregnant state.

Methods: After informed consent a blood sample was taken from a donor by venipuncture and collected in a vacuum tube containing anticoagulant and used in experiments at room temperature (22 °C). All donors were divided in three groups: healthy pregnant women after 22 gestation week and with arterial hypertension and healthy donors. Experiments were carried out on scanning flow cytometer (SFC, fabricated by CytoNova Ltd., Novosibirsk, Russia, http://cyto.kinetics.nsc.ru). This approach allows to investigate RBC gas transport function in terms of diameter, thicknesses, volume, surface area, sphericity index, spontaneous curvature, the rate of anion permeability and the critical relative expansion of a membrane, hemoglobin content.

Results: Total 35 pregnant women and 30 donors were included in this study. Accordidng to obtained data healthy pregnant women have decreased by 32% native rate of anion permeability compare with healthy donors group. However, the ration between native and activated anion permeability among these groups remains the same with accuracy around 6%.

Conclusions: For the first time the mean values and distributions of RBC gas transport fuction was obtained for pregnant state. Such investigation is the small step to diagnostic system which capable of determine the risk of hypoxic state by detailed RBC analysis.

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ISTH Congress Abstracts - https://abstracts.isth.org/abstract/deviations-in-red-blood-cell-gas-transport-function-in-pregnant-state/