Abstract Number: PB1589
Meeting: ISTH 2020 Congress
Background: Von Willebrand disease (VWD) is one of the most common inherited bleeding disorders, caused by quantitative and/or qualitative defect of von Willebrand factor (vWF), a multimeric glycoprotein essential for primary and secondary hemostasis. Conversely, recent studies showed that very high vWF levels might predict in-hospital survival in acute-on-chronic liver failure patients. Diagnosing vWD, based on bleeding history and specific laboratory investigation, may be difficult due to lack of standardization. Numerous assays are available, but measurement of vWF function and plasma concentration is the current practice. A fully automated, immunoturbidimetric vWF:Antigen (vWF:Ag) assay is developed for use on any coagulation analyzer.
Aims: To report diagnostic performance characteristics of this vWF:Ag latex immunoassay on CS-2500.
Methods: LIAPHEN vWF: Ag is an immunoturbidimetric method: vWF:Ag in tested sample reacts with latex particles coupled with rabbit anti-vWF affinity-purified polyclonal antibodies, leading to latex particles agglutination. Performances are evaluated using commercial lyophilized plasma calibrator and controls, and a comparison study with another device is performed on CS-2500 using 100 plasma samples from healthy subjects (n=50), vWD patients (n=25) or liver disease, DIC or sepsis patients (n=25).
Results: Between-run precision coefficient of variation (CV%, n=20), evaluated on lyophilized plasmas with normal and pathological vWF levels, are < 5%. An excellent correlation is obtained with STA-Liatest VWF:Ag on STA-R: y=1.01x-6.61, r=0.993, p< 0.0001. vWF:Ag is well correlated for pathological samples in low range: r=0.971, p< 0.0001 and high range: r=0.983, p< 0.0001.
Conclusions: This vWF:Ag assay is highly sensitive, specific, reproducible and standardized to rapidly assess vWF:Ag concentrations in human citrated plasma. The extended dynamic range is of high interest for the diagnosis of vWD (low levels), as well as for high to very high vWF concentrations, especially for inflammatory diseases and prediction of survival in acute-on-chronic liver failure patients.
To cite this abstract in AMA style:Dunois C, Gueret P, Parage R, Pontis A, Nedelec F, Gouin I, Amiral J. Diagnostic Performances Evaluation of LIAPHEN vWF: Ag on CS-2500 [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/diagnostic-performances-evaluation-of-liaphen-vwf-ag-on-cs-2500/. Accessed January 23, 2022.
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