Abstract Number: PB1255
Meeting: ISTH 2022 Congress
Theme: Platelet Disorders, von Willebrand Disease and Thrombotic Microangiopathies » Blood Cells and Vessel Wall
Background: Venous thromboembolism (VTE) is a multifactorial disease that often occurs in the presence of provoking factors; major risk factors induced provoked VTE (pVTE) whereas weakly provoked (wpVTE) is associated with minor ones. Nevertheless, up to 50% of VTE events are defined as unprovoked (uVTE) as their pathogenesis is still unknown. In this regard, the presence of endothelial alterations deserves to be explored as endothelial dysfunction (ED) is known to trigger thrombus formation. Endothelial colony-forming cells (ECFCs) are the true endothelial progenitors proposed as cell therapy product or liquid biopsy in vascular disorders.
Aims: To investigate whether ECFCs in VTE patients show alterations that may reflect a perturbation of the endothelial compartment involved in uVTE pathogenesis.
Methods: ECFCs were isolated and expanded from peripheral blood mononuclear cells obtained from 40 VTE patients (13 uVTE, 16 wpVTE, 11 pVTE) and 10 matched healthy donors (HDs). ECFCs were analyzed for efficiency of isolation, cell viability and growth. ED was assessed by in vitro functional assays.
Results: ECFCs were isolated from the three subgroups of VTE patients with a similar efficiency as HDs in term of frequency and number of colonies. In pVTE, a trend in the reduction of the frequency of subjects who gave origin to ECFCs was observed. Time of appearance was longer in VTE with the difference being significant only in wpVTE patients. VTE ECFCs showed a high rate of early senescence (passage≤2), with wpVTE group being characterized by the highest frequency.
Conclusion(s): Our results indicate alterations in the growth of VTE ECFCs thus confirming their use to investigate ED role in uVTE pathogenesis. A deeper characterization of ECFCs, still ongoing, will allow the identification of the molecular mechanisms involved in ED in uVTE and to disclose whether they are associated with uVTE only are shared with secondary VTE.
To cite this abstract in AMA style:
Cancellara A, Bacci M, Bertolani L, Romualdi E, Pessi V, Lodigiani C, Donadini M, Della Bella S, Calcaterra F, Mavilio D. Disclosing the role of endothelial dysfunction in the pathogenesis of unprovoked venous thromboembolism by using patient-specific endothelial colony-forming cells [abstract]. https://abstracts.isth.org/abstract/disclosing-the-role-of-endothelial-dysfunction-in-the-pathogenesis-of-unprovoked-venous-thromboembolism-by-using-patient-specific-endothelial-colony-forming-cells/. Accessed March 21, 2024.« Back to ISTH 2022 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/disclosing-the-role-of-endothelial-dysfunction-in-the-pathogenesis-of-unprovoked-venous-thromboembolism-by-using-patient-specific-endothelial-colony-forming-cells/