Abstract Number: PB0461
Meeting: ISTH 2020 Congress
Theme: Diagnostics and OMICs » Biomarkers of Thrombosis and Hemostasis
Background: Dyslipidaemia is common adverse outcomes in Brazilian Renal Transplant Recipients (RTR) which are correlated with an increase in cardiovascular risk, mainly associated with other comorbidities, such as atherosclerosis and thrombotic diseases. Thrombin holds a significant role in haemostasis. Its assessment may be a potential tool for cardiovascular risk stratification.
Aims: This study aims to explore the relation between thrombin generation and dyslipidaemia in RTR.
Methods: In this cross-sectional study, 78 RTR was included, 44 dyslipidaemic RTR and 34 normal lipidic profile RTR (control group). Informed consent was obtained and this study was approved by the local Ethics Committee. Lipidic profile values and other parameters were obtained from medical records. Thrombin Generation test (TGT) was performed by the Calibrated Automated Thrombogram (CAT) method at 1 and 5 pM concentration of tissue factors (TF) trigger in platelet-poor plasma. Lag time, endogenous thrombin potential (ETP), peak and time to peak (TTpeak) were acquired from the thrombin generation curve. Data were presented as mean and standard deviation (Unpaired t-test). Additionally, Spearman´s correlation test was performed. GraphPad Prism and SPSS software were used. P< .050 was significant.
Results: Dyslipidaemic RTR showed higher levels of ETP and peak at 1 pM and 5 pM TF concentrations and decreased TTpeak levels in 1 pM TF concentrations compared than the control group (Figure 1). Lag time and TTpeak were positively correlated with HDL cholesterol (P< .05 for both in 5 pM TF). Triglycerides were positively correlated with ETP and Peak in both TF concentrations (Values of P< .00 for all) (Table 1).
Conclusions: The presence of dyslipidaemia was associated with a procoagulant profile between RTR, which may favour thrombotic diseases and, consequently, allograft rejection and death. The TGT may be a promising tool for the management of renal transplanted recipients, which deserves further investigations.
Support: Capes and CNPq.
[Thrombin generation test in Brazilian Renal Transplant Recipients allocated in groups of dyslipidaemia and control. ]
| Parameters | r | *P |
| ETP 1 pM TF vs Cholesterol total | .247 | .035 |
| ETP 1 pM TF vs Triglycerides | .284 | .015 |
| ETP 1 pM TF vs non-HDL cholesterol | .252 | .038 |
| PEAK 1 pM TF vs Triglycerides | .362 | .002 |
| ETP 5 pM TF vs Triglycerides | .322 | .007 |
| PEAK 5 pM TF vs Triglycerides | .469 | .000 |
| Lag time 5 pM TF vs HDL cholesterol | .259 | .034 |
| TTpeak 5 pM TF vs HDL cholesterol | .280 | .023 |
[Spearman´s correlation assay in renal transplant recipients]
To cite this abstract in AMA style:
Martins S, Alves L, Carvalho MDG, Dusse L, Alpoim P, Mota AP. Dyslipidaemia Associated with Increase of Thrombin Generation in Renal Transplant Recipients [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/dyslipidaemia-associated-with-increase-of-thrombin-generation-in-renal-transplant-recipients/. Accessed January 28, 2022.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/dyslipidaemia-associated-with-increase-of-thrombin-generation-in-renal-transplant-recipients/