Abstract Number: PB0217
Meeting: ISTH 2020 Congress
Background: Vitamin K-dependent coagulation and anticoagulation factors are indispensible proteins that regualte the coagulation process. We report Ca2+and Mg2+binding sites in the Gla domain of factor IX that stabilize the structure of factor IX upon binding Ca2+and Mg2+ions. Physiologic concentrations of Mg2+ions augmented the biological activity of factor IXa, even in the presence of excess concentrations of Ca2+ions. This finding suggests that Mg2+ions also bind to factor IX Gla domain and cause structural changes that contribute to increased activity. In addition to factor IX, several other coagulation and anticoagulation factors (factors VII, X and protein C) are also reported to be affected by the presence of Mg2+and Ca2+ions.
Aims: The contribution of Mg2+and Ca2+to other vitamin K-dependent coagulation factors in membrane-bound processes was evaluated by assessing the affinities of various coagulation factors to phospholipid membranes in the presence of Mg2+and Ca2+ions.
Methods: Here, we report on the effect of Mg2+ion on the interaction of coagulation proteins with phospholipid membranes. Phospholipids were immobilized on a sensor chip L1, and the interactions between coagulation proteins and phospholipid membranes were measured by surface plasmon resonance (SPR) using BIACORE.
Results: Factor IX, factor X, and prothrombin bound to phospholipid membrane in the presence of Ca2+ions in a concentration-dependent manner, and the binding affinities were further increased by adding a physiologic concentration of Mg2+ions.
Conclusions: SPR assay results indicate that Mg2+ions have an effect on binding of the main coagulation factors to phospholipid membranes during blood coagulation. Further investigation is now underway to elucidate the effect of Mg2+ions on the coagulation reaction.
To cite this abstract in AMA style:Shikamoto Y, Morita T, Mizuno H, Mori K. Effect of Mg(II) Ion on the Interaction of Coagulation Factors with Phospholipid Membranes [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/effect-of-mgii-ion-on-the-interaction-of-coagulation-factors-with-phospholipid-membranes/. Accessed February 27, 2024.
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