Abstract Number: PB0763
Meeting: ISTH 2020 Congress
Background: Thrombus formation on a disrupted atherosclerotic plaque is a key event that leads to atherothrombosis.
Aims: The aim of this study was to generate a hamster model of abdominal aorta atherothrombotic stenosis with morphological similarities to the human disease and the subsequent assessment of the reliability of B- mode ultrasound- guided sono- photomechanic combination thrombolytic therapy accompanied by streptokinase- loaded microbubbles administration in this model.
Methods: Briefly, Golden Syrian hamsters were submitted to abdominal aorta atherothrombotic stenosis by primary balloon injury followed by a 1.5% cholesterol- rich diet injury for 12 weeks and finally perivascularly severe cold injury. Then treatment group underwent extracorporeal pulsed- high intensity focused ultrasound (F= 150 KHz, P= 25 W, PD= 250 ms)- mediated inertial cavitation therapy and catheter- based Q-switched Nd: YAG laser (F= 532 nm, P= 25 W, PD= 15 ns)- mediated inertial cavitation therapy accompanied by simultaneously intravenous streptokinase (25000 Units/kg)- loaded PESDA (Perfluorocarbon- Exposed Sonicated Dextrose Albumin) microbubbles (100ml/kg, 2-5 × 105 bubbles/ml) administration, wherein diagnostic B- mode ultrasound is combined with therapy system, with a goal of increased safety.
Results: Results from color Doppler and B-mode ultrasonography and histopathology, showed a significant reduction in the mean value for blood mean velocity and the percentage of luminal cross-sectional area of stenosis and a significant increase in the mean value for blood volume flow at the stenotic region in the treatment group compared with the other groups (p< 0.05).
Conclusions: Enhanced thrombolytic effect of sono- photomechanic combination therapy, induced by inertial cavitation effect of microbubbles can cause to enhance the anti-thrombotic effect of streptokinase and significantly dilate the luminal cross- sectional area of stenosis.
To cite this abstract in AMA style:Mehrad H, Farhoudi M. Effect of Streptokinase-Loaded Microbubbles-Mediated B-Mode Ultrasound-Guided Sono-Photomechanic Combination Thrombolytic Therapy on Atherothrombotic Stenosis [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/effect-of-streptokinase-loaded-microbubbles-mediated-b-mode-ultrasound-guided-sono-photomechanic-combination-thrombolytic-therapy-on-atherothrombotic-stenosis/. Accessed March 3, 2021.
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