Abstract Number: PB0150
Meeting: ISTH 2020 Congress
Background: Hibernating 13-lined ground squirrels (Ictidomys tridecemlineatus) undergo a dramatic drop in temperature and blood flow during torpor. Remarkably, platelets stored in the ground squirrel’s body at temperatures of 4-8°C remain functional when the animal arouses. To avoid stasis clots, both primary and secondary hemostasis are suppressed through mechanisms that remain unclear.
Aims: We aimed to determine how the proteomes of platelets from animals in summer, fall entrance, and winter hibernation differ to in a manner that may contribute to hemostatic adaptation.
Methods: Platelets were isolated from blood collected from five animals in June, October and January. Platelet lysates from each animal were digested and purified to prepare peptides for tandem mass tag (TMT) labeling and quantitative, multiplex mass spectrometry analysis.
Results: Relative to entrance and summer animals, platelets from ground squirrels in hibernation showed increases in 86 proteins, including coagulation regulators, platelet degranulation factors, and apolipoproteins. Platelets from summer non-hibernating animals showed increased levels of 79 proteins enriched in chaperone and transport pathways, and decreases in 48 proteins associated with translation. Platelets from fall animals entering into hibernation showed an increase 27 proteins with few function associations, as well as decreases in 24 proteins involved in complement and coagulation cascades.
Conclusions: Our results suggest that during hibernation, platelets may bind or take up plasma proteins and apolipoproteins. Increases in transport and chaperone proteins in non-hibernator animals may reflect more active and dynamic platelets, while translation is suppressed in the cold, and an increase in translation proteins in platelets from animals either entering or in hibernation could compensate. In fall entrance platelets, a decrease in bound or internal stores of complement and coagulation proteins may protect platelets from becoming activated through repeated rounds of warming and cooling, or ischemic events.
To cite this abstract in AMA style:Cooper S, Cunliffe J, Wilmarth P, David L, Aslan J. Effects of Hibernation on the Platelet Proteome of 13-lined Ground Squirrels [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/effects-of-hibernation-on-the-platelet-proteome-of-13-lined-ground-squirrels/. Accessed May 6, 2021.
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