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Establishing Reference Values in Burkina Faso: A Study of Haemostasis Global Assays and Coagulation Factors Eight and Nine, Based on Blood Donors

1University Joseph KI-ZERBO, UFR SDS, Fundamental Sciences Department, Laboratory of Haematology, Ouagadougou, Burkina Faso, 2National Blood Centre, Ouagadougou, Burkina Faso, 3University Joseph KI-ZERBO, Ouagadougou, Burkina Faso, 4Yalgado Ouedraogo Teaching Hospital, Ouagadougou, Burkina Faso, 5Paediatric Teaching Hospital Charles de Gaulle, Ouagadougou, Burkina Faso, 6University Nazi Boni, National Institute for Medical Sciences, Bobo-Dioulasso, Burkina Faso, 7Souro Sanou Teaching Hospital, Bobo-Dioulasso, Burkina Faso, 8Necker Enfant Malade Teaching Hospital, Laboratory of General Hematology, Paris, France, 9University Cheikh Anta Diop, Dakar, Senegal

Abstract Number: PB0393

Meeting: ISTH 2021 Congress

Theme: Diagnostics and OMICs » Laboratory Diagnostics

Background: The need to explore haemostasis is increasing in Burkina Faso with the changing health profile in the country, where, cardiovascular and metabolic diseases are emerging. However, interpretation of the results faces the absence of duly established reference values adapted to local populations.

Aims: Our study aims to establish reference values for haemostasis global Assays (PT, aPPT, Fibrinogen) and FVIII and FIX levels.

Methods: We carried out a cross-sectional study from August 31 to October 22 2020 in the regional blood transfusion centre of Ouagadougou. Healthier Voluntary blood donors were included following CLSI guidelines. The sample size was 280 (140 males and 140 females). Inclusion criteria were, no current disease, no medical history or laboratory finding that can influence coagulation (smoking, inflammatory diseases, extreme obesity). A 5ml blood sample was drawn out in a citrated tube, centrifuged and the plasma been stored within 4 hours at minus 80°C. Later, we performed assays comprising PT, aPPT fibrinogen, FIX for all 280 subjects, and FVIII for only 140. The Sysmex™ CA660 coagulometer was used with Siemens™ reagents. Reference values were calculated using “Mean ± 2X SD” or the “Central 95 percentile” when appropriate.

Results: Reference values were found to be [1.533 – 3,573] for fibrinogen, [67.72 – 116.57] for PT, [19.86 s – 28.74 s] for aPTT, [45.09% – 149.4%] for FIX, and [46.0% – 174.6%] for FVIII. FVIII was significatively lower in blood group O subjects compared to non-O blood groups (74.8% to 93.5%; p = 0.005). Fibrinogen was higher in female than in male (2.81 g/L versus 2.30 g/L, p < 0.01).

Conclusions: The study demonstrates it is necessary to establish Reference values that fit local populations as lower limits of FVIII and FIX reference intervals appears to be low than expected and closer to minor haemophilia state.

To cite this abstract in AMA style:

Nebie K, Sawadogo S, Koulidiati j, Ouedraogo W, Kima D, Nikiema M, Sawadogo S, Lasne D, Toure A, Kafando E, GIH Study Group . Establishing Reference Values in Burkina Faso: A Study of Haemostasis Global Assays and Coagulation Factors Eight and Nine, Based on Blood Donors [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/establishing-reference-values-in-burkina-faso-a-study-of-haemostasis-global-assays-and-coagulation-factors-eight-and-nine-based-on-blood-donors/. Accessed July 1, 2022.

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