Abstract Number: PB0577
Meeting: ISTH 2020 Congress
Background: Benzylsulfonyl-D-Arg-Pro-4-amidinobenzylamide (BAPA) is a potent inhibitor of thrombin and factor Xa. BAPA has previously been described as an alternative to citrate or hirudin anticoagulants for the collection of blood samples to be used for platelet function studies.
Aims: Using the BAPA tube for T-TAS01, to 1) compare the performance of BAPA anticoagulated blood samples to citrate and hirudin anticoagulated blood samples using light transmittance aggregometry and T-TAS PL measurements, respectively, and 2) to determine the stability of BAPA anticoagulated whole blood samples.
Methods: Blood samples were collected from healthy volunteers with or without aspirin into vacuum tubes containing the anticoagulant to be evaluated. With the exception of the sample stability determination, all measurements were performed within 3 hours after sample collection using the manufacturer’s recommended procedures. LTA measurements were performed using 5 µM ADP, 2 mM arachidonic acid, 2 µg/mL collagen and 4 µg/mL collagen.
Results: Results from the comparison between BAPA and citrate using N = 22 matched samples yielded average percent differences of 3%, 4%, 1%, and 3% for ADP, arachidonic acid, 2 µg/mL collagen and 4 µg/mL collagen, respectively. Both tubes also correctly identified two donors that were taking medications that affect AA and collagen-induced aggregation. Using the T-TAS PL assay, results from N = 9 matched BAPA and hirudin samples were highly correlated, with r2 = 0.95, and a Deming regression slope = 1.00 and intercept = -19.3 (all not significantly different from y = x). Using T-TAS PL assay measurements of N = 4 samples, BAPA blood samples exhibited no significant decay within 8 hours after sample collection.
Conclusions: The highly comparable performance and superior sample stability suggest that BAPA is a suitable alternative to citrate and hirudin for anticoagulation of blood samples to be used for platelet function studies.
To cite this abstract in AMA style:
Dahlen J, Bliden K, Tantry U, Gurbel P, Dobashi K, Hosokawa K. Evaluation of a New Benzylsulfonyl-D-Arg-Pro-4-Amidinobenzylamide (BAPA) Blood Collection Tube for Platelet Function Studies [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/evaluation-of-a-new-benzylsulfonyl-d-arg-pro-4-amidinobenzylamide-bapa-blood-collection-tube-for-platelet-function-studies/. Accessed March 21, 2024.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/evaluation-of-a-new-benzylsulfonyl-d-arg-pro-4-amidinobenzylamide-bapa-blood-collection-tube-for-platelet-function-studies/