Abstract Number: PB0884
Meeting: ISTH 2021 Congress
Theme: Platelet Disorders, von Willebrand Disease and Thrombotic Microangiopathies » Platelet Function Disorders, Acquired
Background: Abnormalities of platelet function are associated with an increased risk of bleeding. Light transmission aggregometry (LTA) is the gold standard and the most widely used laboratory method to screen patients with suspected abnormalities of primary hemostasis. LTA is a time-consuming and technically challenging method, so that an automated LTA-based method for use on Sysmex CS-2400, a high-performance automated coagulation analyzer, has been recently developed.
Aims: To evaluate the diagnostic capacity of CS-2400 with Revohem aggregation reagents panel for the diagnosis of platelet disorders and for monitoring the effect of anti-platelet therapy.
Methods: Platelet aggregation induced by adenosine diphosphate (ADP), arachidonic acid (AA), collagen and epinephrine was performed according to ISTH guidelines, on Sysmex CS-2400 analyzer and on lumi-light-transmission aggregometry (Chrono-log,Mascia Brunelli, IT).
Light transmission was recorded and aggregation response to all agonists was analysed and compared to controls.
To evaluate platelet response in patients on anti-platelet therapy, we used the “Platelet Aggregation Level (PAL)” on CS-2400, which decreases with platelet aggregation activity reduction. A-PAL and C-PAL were calculated from the wave obtained using two concentrations of ADP and collagen respectively.
Results: Ten patients with primary secretion defects (PSD), 11 patients on anti-platelet therapy and 10 healthy controls were enrolled in the study.Patients with PSD showed reduced median of % light-transmission compared to controls using each agonist. Data obtained with CS-2400 showed a low intra-group variability for each agonist except epinephrine (Figure 1).
Figure 1. % of light transmission in control group and PSD patients. Distribution of % of light transmission according to different groups of patients. The box plots represent the interquartile ranges, the solid horizontal line inside each box plot is the median value and the vertical bars delimit the minimum and maximum values of the distribution. The black circles identify outliers.
Patients on anti-platelet therapy showed statistically significant reduced (p=0.001) median of % light-transmission compared to controls with ADP 4-20μM and AA 1mM. In these patients, A-PAL and C-PAL scores were lower than controls (Figure 2).
Figure 2. % of light transmission in control group and patients on anti-platelet therapy (panel A). C-PAL and A-PAL scores in control group and patients on anti-platelet therapy (panel B). Distribution of % of light transmission (Panel A) and PAL score (panel B) according to different groups of patients. The box plots represent the interquartile ranges, the solid horizontal line inside each box plot is the median value and the vertical bars delimit the minimum and maximum values of the distribution. The black circles identify outliers
Conclusions: Milan preliminary data using CS-2400 analyzer showed a good diagnostic capacity for PSD patients and a good performance in evaluating the aggregation response in patients on anti-platelet therapy.
To cite this abstract in AMA style:
Lecchi A, La Marca S, Padovan L, Boscarino M, Artoni A, Arai N, Shinohara S, Capecchi M, Peyvandi F. Evaluation of an Automated Platelet Aggregation Method for the Detection of Patients with Congenital or Acquired Platelet Function Defects [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/evaluation-of-an-automated-platelet-aggregation-method-for-the-detection-of-patients-with-congenital-or-acquired-platelet-function-defects/. Accessed December 7, 2021.« Back to ISTH 2021 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/evaluation-of-an-automated-platelet-aggregation-method-for-the-detection-of-patients-with-congenital-or-acquired-platelet-function-defects/