Abstract Number: PB0080
Meeting: ISTH 2020 Congress
Theme: Arterial Thromboembolism » Cardiovascular Risk Factors
Background: Contact activation is an essential aspect of biocompatibility. It plays a role in blood collection because of the material of the tubes and can cause a low-grade coagulation activation. For airborne / urban particulate matter it may be a factor in causing lung disease and other health problems.
Aims: We tested laboratory materials, air particulate matter for causing contact activation in plasma.
Methods: Kallikrein generation and NAPTT in plasma. Aerosil was the calibrator
Results: In the kallikrein generation, some of the laboratory material showed strong reactions (Eppendorf tubes, Greiner Elisa, Nunc High Bind and Coater black plates) and were excluded. Various APTT- silica reagents were tested and had variable potency: 336-696 µg/ml aerosil-equivalents: the highest was Synthasil. Actin FS showed 135 ug/ml aerosil-equivalents. For solutions with 400 µg/ml: asbestos was equivalent to 169 µg/ml aerosil-eq; Cerium oxide and diesel exhaust to 75 µg/ml aerosil-eq and Beach sand dust to 70 µg/ml aerosil-eq. Air particulate matter was harvested on building surfaces and roadsides and mixed with fluid to 400 µg/ml. Along roads the potency was around 3-7 µg/ml aerosil-eq. with a peak of 19 and trough of 1,4 µg/ml aerosil-eq. All particles were tested after uptake in water and tested for size distribution by dynamic light scattering: all were between 200 -700 nm hydrodynamic radius. Comparing contact activation of kallikrein with activation of factor XI showed that some air borne particles were inactive in factor XIa activation. When both kallikrein and factor XIa were formed the potency showed a good correlation.
Conclusions: Airborne pollution particles have a range of potencies to activate the contact system, and specific activation of kallikrein is possible. It suggests that next to number, also quality of the particles may be relevant for inducing health problems.
To cite this abstract in AMA style:
Kluft C, van Leuven K. Evaluation of Biological Effects of Airborne / Urban Pollution Particles [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/evaluation-of-biological-effects-of-airborne-urban-pollution-particles/. Accessed March 21, 2024.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/evaluation-of-biological-effects-of-airborne-urban-pollution-particles/