Abstract Number: PB0363
Meeting: ISTH 2021 Congress
Background: Endothelial cells form the barrier between vessels and tissue and control the flow of substances and fluid into and out of a tissue. Endothelial dysfunction is a type of non-obstructive artery disease in which the blood vessels constrict instead of dilating.
Aims: In this study, we developed an experimental near- infrared laser- mediated photobiomodulation therapy system, and investigated its effectiveness on inflammation reduction and improvement of endothelial dysfunction in the hamster common carotid artery model of early stage atherosclerosis, using a new computerized ultrasound image processing software.
Methods: Briefly, golden Syrian hamsters’ right common carotid arteries in the treatment group, treated using extracorporeal near- infrared laser- mediated photobiomodulation therapy system (λ= 850 nm, P= 150 mW). In order to evaluate endothelial-dependent relaxation, acetylcholine-mediated dilation (AMD) was measured during the infusion of acetylcholine at a rate of 0.5 μg/kg/min and endothelial independent relaxation was evaluated by measuring nitroglycerin mediated dilation (NMD) during the infusion of nitroglycerin at a rate of 5μg/kg/min. For off-line analysis, a computerized analysis method for evaluating instantaneous changes in the wall of the hamster carotid artery was used. For automatic measurement of the mean wall thickness and the percentage of luminal cross-sectional area of stenosis from longitudinal B-mode ultrasound images, two algorithms, i.e., maximum gradient and dynamic programming were composed and implemented. Reference points and cost function were based on dynamic programming and maximum gradient, respectively.
Results: Results from computerized B- mode ultrasound image processing software data showed significant differences in AMD between the treated and the non-treated hamsters (p<0.05), whereas there were no significant differences in NMD between the treatment and normal groups (p>0.05).
Conclusions: It is concluded that the new automatic method enables accurate and repeated evaluation of improvement of endothelial function and regression of early stage atherosclerosis using near- infrared laser- mediated photobiomodulation therapy in this animal model.
To cite this abstract in AMA style:Mehrad H, Farhoudi M. Evaluation of Effect of Extracorporeal Near-infrared Laser-mediated Photobiomodulation Therapy on Endothelial Dysfunction Improvement Using a New B-mode Ultrasound Image Processing Software-based Automatic Method [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/evaluation-of-effect-of-extracorporeal-near-infrared-laser-mediated-photobiomodulation-therapy-on-endothelial-dysfunction-improvement-using-a-new-b-mode-ultrasound-image-processing-software-based-auto/. Accessed July 1, 2022.
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