Abstract Number: PB0640
Meeting: ISTH 2020 Congress
Background: Platelet concentrates (PCs) are widely used for the treatment of thrombocytopenia and active platelet-related bleeding. However, detailed pharmacological efficacy of PCs to improve hemostatic ability is not fully understood.
Aims: To comparatively evaluate platelet reactivity for specific agonists and overall thrombogenicity of apheresis-collected PCs.
Methods: Three different apheresis-collected PCs were provided by Japanese Red Cross. Agonist-inducible platelet aggregation was evaluated by light transmittance aggregometry (LTA) and Multiplate, and total thrombus-formation analysis system (T-TAS) was used to evaluate thrombogenicity under flow conditions inside a microchip coated with collagen and tissue thromboplastin.
Results: White thrombi comprising activated platelets and fibrin fibers were formed inside the T-TAS microchip, and the occlusion time (OT) due to thrombus formation was 8.1±0.9 min. Thrombus formation was efficiently suppressed by SCH79797 (PAR-1-antagonist), OS-1 (GPIbα-antagonist) and abciximab (GPIIbIIIa-antagonist) in a dose dependent manner, whereas the effect of aspirin (100 µM) and AR-C66096 (P2Y12-antagonist; 1 µM) were limited even in combination (OT; 8.5±1.1 min).
Platelet aggregation induced by ADP and ristocetin was significantly impaired in the PC samples, whereas collagen and TRAP-6 induced aggregation was relatively preserved. Maximum aggregation of LTA with ADP (6.5 µM), ristocetin (0.77 mg/mL), collagen (3.2 µg/mL) and TRAP-6 (32 µM) were 13.0±6.9, 2.0±0.0, 50.8±9.3 and 42.1±16.0, and Multiplate-AUC were 1.4±1.4, 41.4±13.5, 100.7±2.9 and 66.9±11.1, respectively.
Conclusions: Platelet activation mediated by subendothelial collagen and thrombin might be important for PCs to achieve hemostasis, and thrombogenicity analysis by T-TAS might be a useful tool for assessing the functional hemostatic property of PCs.
To cite this abstract in AMA style:Nagasato T, Ohnishi-Wada T, Sameshima-Kaneko H, Oyamada C, Hosokawa K, Matsubara Y. Evaluation of Thrombogenicity of Apheresis-collected Platelet Concentrates under Blood Flow Condition [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/evaluation-of-thrombogenicity-of-apheresis-collected-platelet-concentrates-under-blood-flow-condition/. Accessed September 24, 2023.
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