Abstract Number: PB0375
Meeting: ISTH 2020 Congress
Theme: Coagulation and Natural Anticoagulants » Microparticles
Background: Cirrhosis is frequently associated to thromboembolic events particularly in portal vein. Moreover microparticles are considered as prothrombotic state biomarkers in many clinical diseases.
Several studies have evaluated the expression of prothrombotic microparticles in cirrhotic patients but there is not enough data regarding their prothrombotic potential.
Aims: To evaluate the procoagulant activity expressed by circulating microparticles in cirrhotic patients and to correlate this activity with clinical and biological status.
Methods: A comparative case-control study was conducted in 32 cirrhotic patients and 14 controls. Laboratory tests included levels of both FVIII and PC and thrombin generation assay. Thrombin generation test (TGT) in platelet poor plasma was performed using 3 reagents from STAGO: PPP reagent low (R1), PRP reagent (R2) and MP reagent (R3). TGT parameters analyzed were: lag time, time to peak , Peak, endogenous thrombin potential (ETP) and velocity index. The ratio FVIII/PC was calculated to evaluate the hemostatic balance. TGT results were expressed originally as ratios: R2/R1 and R3/R1 to assess respectively the contribution of endogenous phospholipids and tissue factor.
Results: The mean age was 65.3 years old [35 and 90] and the sex ratio was 1. Patients were classified using CHILD-PUGH score as following: CHILD A (15 patients), CHILD B (10 patients) and CHILD C (7 patients). FVIII/PC ratio was significantly higher in cirrhotic patients than in controls (4.6 vs 1.14; p< 0.001). The ratio ETP (R3/R1) was significantly increased in controls compared to patients (1 vs 0.95; p=0.038). Whereas, ETP (R2/R1) was not significantly different in the two groups (1.14 vs 1.02; p=0.262). The other TGT parameters were also not significantly different in both groups. The ratio FVIII/PC was not significantly correlated to ETP ratios.
Conclusions: Although hemostatic balance evaluated with the ratio FVIII/PC reflected hypercoagulable state, micropaticle procoagulant activity assessed by TGT was not increased in cirrhotic patients.
To cite this abstract in AMA style:
Mejda B, Hela B, Dhouha B, Asma L, Boubaker J, Neila BR. Expression of Circulating Microparticles Procoagulant Activity in Cirrhotic Patients [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/expression-of-circulating-microparticles-procoagulant-activity-in-cirrhotic-patients/. Accessed September 22, 2023.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/expression-of-circulating-microparticles-procoagulant-activity-in-cirrhotic-patients/