Abstract Number: PB0111
Meeting: ISTH 2021 Congress
Theme: Coagulation and Natural Anticoagulants » Microparticles
Background: Tissue´s calcification, particularly in the cardiovascular system, decreases the elasticity of the blood vessels and favors valvular disease, mainly the aorta.
It increases with age, chronic kidney or inflammatory disease.
There are many possible mechanisms in the calcification process, including vascular smooth muscle cell (VSMC) instability, degradation of calcium, phosphate and elastin, differentiation of osteoclasts, and apoptosis.
The Mps are structures whose size ranges between 50 and 1000 nm and are horizontal vectors of different molecules including proteins, nucleic acids. They are involved in several pathological processes and the evidence is increasing in the participation of the Mps in the calcification of aortic valve.
Aims: To identify the presence of minerals in the Mps from patients undergoing primary aortic valve replacement.
Methods: We studied the presence of calcium in the Mps of 3 individuals undergoing primary replacement of the aortic valve at the National Institute of Cardiology. The protocol was approved by the Research and Ethics Committee with No. 18-1043 and the individuals signed an informed consent. Mps were obtained by the ultracentrifugation method, the concentration was obtained by determining the proteins and was expressed as mg/mL. The Mps were analyzed in a scanning microscope Jeol JSM-7401F (Field Emission Scanning Electron Microscope), with an excitation potential of 10KeV, for microanalysis during 300 seconds in an area of 200 nm.
Results: A)
Figure 1. A) As a proof of concept result, it shows the image obtained from a sample that corresponded to a 56-year-old male adult. B) Presence of various minerals is appreciated, such as Ca, Na, Si, S, Cl, Fe including trazes of Mg, Al, P, Ag, Au, K and Ti.
B)
Conclusions: Our results show the presence of minerals in Mps of patients with aortic valve stenosis.
To cite this abstract in AMA style:
Flores Garcia M, De la Peña Diaz A, Soria Castro E, Peña Duque M, Hernández López JR, Reyes Munguía D, López Granados M, Villegas Hernández JC. Extracellular Microparticles as Aortic Valves Calcification Vectors, Proof of Concepts [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/extracellular-microparticles-as-aortic-valves-calcification-vectors-proof-of-concepts/. Accessed June 25, 2022.« Back to ISTH 2021 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/extracellular-microparticles-as-aortic-valves-calcification-vectors-proof-of-concepts/