Abstract Number: PB2540
Meeting: ISTH 2020 Congress
Background: Preeclampsia (PE) is associated with endothelial dysfunction, which can trigger abnormal activation of hemostasis.
Aims: To evaluate prothrombin fragment 1+2 (F1+2) levels, an indicator of thrombin generation, as a potential marker for prognosis and monitoring of PE, compared to D-dimer (DDi).
Methods: Ethical aspects have been observed previously (CAAE 0530.0.203.00-09). A total of 59 pregnant women participated in the study, including 19 with severe PE, 22 with mild PE and 18 normotensive pregnant women (controls). F1+2 and DDi levels were determined by Elisa. Statistical analysis was performed by ANOVA and P< 0.05 was considered significant.
Results: No difference was found for F1+2, comparing the average values for women with severe PE (2.50±1.07nmol/L), mild PE (2.45±0.85nmol/L) and controls (2.94±1.09nmol/L). Conversely, measurement of DDi levels revealed a marked exacerbation of coagulation in severe PE (1497.8±435.3ng/mL) compared to mild PE (1060.3±259.2ng/mL) and controls (1146.6.8±311.2ng/mL) (Dusse et al., Clin Chim Acta. 2013,415:226-9).
Conclusions: According to DDi levels results, an increase conversion of prothrombin into thrombin resulting in higher levels of F1+2, was expected in severe PE. However, it was not obtained. Our results are in line with a previous one (Cadroy et al., Br J Obstet Gynaecol. 1993,100(5):416-20) who did not observe an increase of F1+2 in PE. Therefore, accuracy of F1+2 determination to assess thrombin generation has been questioned. According to Cadroy et al., some F1+2 molecules are retained on the catalytic phospholipid surface where they were formed, not being released into the circulation. It results in underestimated plasma levels of F1+2. Concluding, F1+2 levels, unlike DDi, failed as a potential hemostatic marker for prognosis and monitoring of PE.
Support: CNPq (404353/2016-9) and FAPEMIG/BRAZIL (PPM-00764-16)
To cite this abstract in AMA style:Dusse L, Alpoim P, Carvalho M. F1+2 in Preeclampsia: Is it a Good Hemostatic Marker? [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/f12-in-preeclampsia-is-it-a-good-hemostatic-marker/. Accessed August 16, 2022.
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