Abstract Number: PB1845
Meeting: ISTH 2020 Congress
Background: Misfolding of extracellular proteins, induced by environmental factors as mechanical stress, glycation, oxidative stress, proteolysis and electrophilic stress leads to activation of platelets. Lipid rafts, ganglioside- and cholesterol enriched membrane microdomains directly promote aggregate formation of misfolded proteins.
Aims: As the general anaesthetic propofol is formulated in a lipid emulsion and as fat emulsions are used for parenteral nutrition in intensive care patients, we studied the effect of such formulations on the activation of platelets induced by HOCl modified proteins.
Methods: Platelet function was analysed using flow cytometry, adhesion assays, aggregation, procoagulant activity and confocal laser scanning microscopy.
Results: We observed a strong enhancement of platelet activation induced by HOCl modified albumin in all used assays with lipid formulated propofol and clinically relevant concentrations. Pure propofol without lipids had no effect. In addition, Lipofundin MCT 20 showed the same enhancing effects as lipid formulated propofol.
Conclusions: It is likely that the fat emulsions induced stronger platelet activation by enhancing the aggregate formation of the misfolded albumin, leading to enhanced crosslinking of platelet receptors and hence stronger activation. Infusion of propofol and any other lipid-based drug or parenteral nutrition might have an influence on prothrombotic tendency under inflammatory conditions.
To cite this abstract in AMA style:Herminghaus AG, Brodde MF, Kehrel BE. Fat Emulsion for Parental Nutrition as Well as Lipid Emulsion of the Drug Propofol Strongly Enhance Platelet Activation Induced by Human Albumin, Misfolded by Structural Modification with the Granulocyte Defence Weapon HOCl [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/fat-emulsion-for-parental-nutrition-as-well-as-lipid-emulsion-of-the-drug-propofol-strongly-enhance-platelet-activation-induced-by-human-albumin-misfolded-by-structural-modification-with-the-granuloc/. Accessed May 6, 2021.
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