Abstract Number: VPB1297
Meeting: ISTH 2022 Congress
Background: Circulating levels of selected miRNAs open up new avenues for the identification of more effective and specific biomarkers to identify and risk-stratify stroke patients.
Aims: Aim is to identify and select specific miRNAs to be used as disease biomarkers to improve both prognosis and prediction.
Methods: 48 patients with embolic stroke of undetermined source (ESUS) were involved in the study. We divided the patient groups based on patients who had a second stroke or TIA and did not have. Total RNA was extracted from plasma and quality was assessed with fluorometric electrophoresis. Microarray analysis by Affymetrix. Statistical analysis: in TAC software and R using Signal information obtained from the TAC output, FDR correction, logistic regression, calculated AUC using ROCp R package. Coexpression analysis to identify genes authentically expressed was performed using Spearman correlation (cutoff=0.9, Rpval=0.05).
Results: MiR-4786, miR-1205, miR-548ar-3p, miR-518e-3p were found the most differentially expressed miRNAs between the groups (patients with one stroke vs multiple strokes). miR-4786 was studied only in patients with acute leukemia. Several studies showed the importance of miR-1205 in cell carcinoma and ovarian cancer progression. Moreover, so far only one study showed the regulation of miR-548ar-3p in breast cancer. Finally only one study showed the alteration of miR-518e-3p in Parkinson’s disease patients. Our enrichment analysis showed IL-2 signaling pathway, lipid and lipoprotein metabolism, BDNF signaling pathway, MAPK signaling pathway, Intellectual Disability, Alzheimer’s Disease are significantly related to ESUS- patients.
Conclusion(s): Our results identified several novel circulating miRNAs that are down- or up-regulated in ESUS-stroke patients, as showing the predictive significance for the assessment of risk of the second stroke. Among those with the most relevant differential expression, several miRNAs were already identified to play a role in the pathophysiology of neurovascular diseases, paving the way to a new class of smart pathophysiology-based biomarkers in stroke.
To cite this abstract in AMA style:Eyileten C, Wicik Z, Jarosz-Popek J, Nowak A, Shahzadi A, Keshwani D, Adem T, Mirowska-Guzel D, Pare G, Postula M, Wolska M. Fingerprint of novel circulating microRNAs identify patients with stroke-embolic stroke of undetermined source [abstract]. https://abstracts.isth.org/abstract/fingerprint-of-novel-circulating-micrornas-identify-patients-with-stroke-embolic-stroke-of-undetermined-source/. Accessed February 29, 2024.
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