Abstract Number: PB0425
Meeting: ISTH 2020 Congress
Background: Establishing the relationship between polymorphic genes and phenotypic manifestations of thrombophilia in breast cancer is an essential condition for establishing key pathogenesis links that affect certain physiological functions, through which the risk of thrombosis is formed.
Aims: To study the association of complex carriage of folate metabolism polymorphism with the level of homocysteine in the blood serum of patients with breast cancer.
Methods: The study included 122 patients with breast cancer aged 56 ± 10.0 years. The diagnosis is histologically confirmed. The control group consisted of 142 conditionally healthy women of the Transbaikal Territory at the age of 40.2 ± 9.5 years without a history of cancer at the time of blood sampling. The determination of serum homocysteine level was carried out by high performance liquid chromatography according to A.A. Zhloba and E.L. Blashko (2004). Genotyping for the detection of polymorphism MTHFR (C677T), MTHFR (A1298C), MTR (A2756G), MTRR (A66G) was carried out by polymerase chain reaction in real time. Statistical analysis was performed using Ms Excel 10.0, Statistica 6.0., MDR v.3.0.2. The significance of differences was determined by the Mann-Whitney test and the median and percentiles (25th and 75th) were calculated.
Results: All possible combinations of polymorphic variants of folate metabolism genes were analyzed in patients with sporadic breast cancer and in the comparison group. Combinations MTR2756AG x MTRR66AG and MTHFR677CT x MTHFR1298AA x MTR2756AG x MTRR66AG were found to be associated with a higher concentration of homocysteine in serum compared to carriers of combinations of alternative genotypes and in the control (p < 0.05).
Conclusions: The additive effect of polymorphism of folate metabolism genes, leading to hyperhomocysteinemia, has an independent effect in the formation of the “pathogenetic profile” of thrombophilia due to synergistic interaction, which contributes to the phenotypic realization of thrombosis, a serious complication in breast cancer patients.
To cite this abstract in AMA style:Markovsky A. Gene-Gene Interactions of Folate Cyclein as a Potential Factor of the Risk of Thrombosses in Patients with Breast Cancer [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/gene-gene-interactions-of-folate-cyclein-as-a-potential-factor-of-the-risk-of-thrombosses-in-patients-with-breast-cancer/. Accessed December 3, 2021.
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