Abstract Number: PB1655
Meeting: ISTH 2020 Congress
Background: Studies on platelet function in children older than neonatal period are few and their results are controversial. There are reports of both hypo- and hyper-reactivity in pediatric platelets compared to adults.
Aims: The aim of our study was to compare platelet function in children aged from 1 year to 18 years old with adults.
Methods: Venous blood was collected into vacuum plastic tubes with sodium citrate, final concentration 3.8%. 20 µL of blood was diluted 1:20 in HEPES-buffered Tyrode buffer. For platelet activation, a mixture of collagen-related peptide (1.25 µg/mL), PAR-1 activating peptide SFLLRN (12.5 µM) and 2.5 mM CaCl2 was used. We determined forward scatter (FSC), side scatter (SSC), CD42b-PE, CD61-PE, CD62P-Alexa Fluor 647, PAC-1-FITC, annexin V-Alexa Fluor 647 binding and mepacrine release levels.
Results: Children were divided into 3 age groups: 1 to 5 years old (n=16), 6 to 10 years old (n=23) and 11 to 18 years old (n=32); the control was healthy adults aged 19 to 40 years old (n=49). Procoagulant platelets level in all children groups was 1.5-fold higher than in adults, both in resting and activated states. Resting platelets of children aged 1 to 5 years old had 1.3-fold fewer dense granules than adults’ platelets and lower relative degranulation. Pediatric platelets from all groups had 1.2-fold lower CD62p after activation than adults. CD42b and CD61 on stimulated platelets of children were higher than in adults. No difference between children and adults was found for PAC-1 in resting and activated states, CD42b, CD61 and CD62p in resting state.
Conclusions: Pediatric platelets are characterized by lower granule release and higher procoagulant response than adult platelets, with the same integrin level.
To cite this abstract in AMA style:Ponomarenko E, Ignatova A, Hismatullina R, Shcherbina A, Larin S, Maschan A, Novichkova G, Panteleev M. Healthy Pediatric Platelets are Moderately Hyporeactive But Hyper-Coagulant within a Wide Age Range [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/healthy-pediatric-platelets-are-moderately-hyporeactive-but-hyper-coagulant-within-a-wide-age-range/. Accessed November 30, 2021.
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