Abstract Number: OC 50.2
Meeting: ISTH 2021 Congress
Background: Hypercoagulability, complement activation and endothelial perturbation characterize sever COVID-19. After disease remission, a proportion of convalescent subjects still experience post-COVID-19 symptoms. No information is available on persistence of hemostatic alterations in this setting. Bergamo city, represents one of the first and most affected area by SARS-CoV-2 infection in the world. For this reason, since the beginning we were actively involved in recruiting CCP donors.
Aims: In a large cohort of CCP donors, we aimed to characterize biomarkers of hypercoagulability and of endothelial perturbation in order to find associations with disease severity, demographic characteristics, and anti-SARS-CoV-2 antibody levels.
Methods: Candidate CCP donors were tested for the anti-SARS-CoV-2 antibodies, including anti-S IgG antibodies (Anti-S Ab) and anti-N IgG antibodies (Anti-N Ab). In addition, the following plasma biomarkers were assessed: fibrinogen, protein C, protein S, factor V, factor VIII, factor XIII, D-dimer, and von Willebrand factor (vWF).
Results: 425 CCP candidates (275M/150F, age range 19-67 years) were admitted to donation. Male gender, age > 40 years, and severe form of COVID-19 were identified as independent predictors of high levels of both anti-S and anti-N Ab (p<0.001).
Among hemostatic parameters, levels of vWF antigen, vWF activity and protein C were significantly higher in CCP donors who had severe COVID-19 compared to donors who had non-severe COVID-19 (p<0.001). Furthermore, vWF levels positively correlated with anti-S Ab levels (vWF-antigen r=0.216; vWF-activity r=0.257 and vWF-RiCof r=0.226, p<0.01).
Conclusions: Our data show that gender, age and severe disease can be predictors of an increased immunological response. Furthermore, convalescent subjects show a persistently high vWF levels, suggesting a persistence of the endothelial activation, despite of clinical disease remission.
To cite this abstract in AMA style:Tartari CJ, Milani G, Salvetti F, Marchetti M, Russo L, Vignoli A, Vitali M, Barcella L, Cefis M, Gomez-Rosas P, Schieppati F, Ravasio G, Castellani L, Marchesi G, Sanga E, Romeo D, Falanga A. Hemostatic Profile of COVID-19 Convalescent Plasma (CCP) Donors from Bergamo Area [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/hemostatic-profile-of-covid-19-convalescent-plasma-ccp-donors-from-bergamo-area/. Accessed November 27, 2021.
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